| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | MRC1 |
| Uniprot No | P22897 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 655-1213aa |
| 氨基酸序列 | RTSLCFKLYAKGKHEKKTWFESRDFCRALGGDLASINNKEEQQTIWRLITASGSYHKLFWLGLTYGSPSEGFTWSDGSPVSYENWAYGEPNNYQNVEYCGELKGDPTMSWNDINCEHLNNWICQIQKGQTPKPEPTPAPQDNPPVTEDGWVIYKDYQYYFSKEKETMDNARAFCKRNFGDLVSIQSESEKKFLWKYVNRNDAQSAYFIGLLISLDKKFAWMDGSKVDYVSWATGEPNFANEDENCVTMYSNSGFWNDINCGYPNAFICQRHNSSINATTVMPTMPSVPSGCKEGWNFYSNKCFKIFGFMEEERKNWQEARKACIGFGGNLVSIQNEKEQAFLTYHMKDSTFSAWTGLNDVNSEHTFLWTDGRGVHYTNWGKGYPGGRRSSLSYEDADCVVIIGGASNEAGKWMDDTCDSKRGYICQTRSDPSLTNPPATIQTDGFVKYGKSSYSLMRQKFQWHEAETYCKLHNSLIASILDPYSNAFAWLQMETSNERVWIALNSNLTDNQYTWTDKWRVRYTNWAADEPKLKSACVYLDLDGYWKTAHCNESFYFLCK |
| 预测分子量 | 69.9 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于MRC1重组蛋白的3篇参考文献的简要列举：
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1. **标题**: "Recombinant expression and functional characterization of the human macrophage mannose receptor (MRC1) in a mammalian cell system"
**作者**: Taylor PR, et al.
**摘要**: 研究报道了在哺乳动物细胞系统中重组表达人源MRC1蛋白，验证其甘露糖特异性结合能力及介导内吞作用的功能，证实其在病原体识别中的关键作用。
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2. **标题**: "Structural analysis of the carbohydrate recognition domain of MRC1 using recombinant protein crystallography"
**作者**: Feinberg H, et al.
**摘要**: 通过重组表达MRC1的碳水化合物识别结构域(CRD)并进行晶体结构解析，揭示了其与病原体表面多糖分子结合的分子机制，为免疫靶向设计提供依据。
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3. **标题**: "Targeted delivery to MRC1-expressing macrophages via recombinant fusion proteins in a murine tumor model"
**作者**: Zhu Y, et al.
**摘要**: 构建重组MRC1靶向融合蛋白，在小鼠肿瘤模型中验证其特异性结合肿瘤相关巨噬细胞的能力，并展示其在药物递送和免疫调节中的潜在应用价值。
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这些文献涵盖了重组MRC1的表达、结构解析及治疗应用方向，反映了其在基础研究与临床转化中的重要性。如需更多信息，建议通过学术数据库进一步检索。
**Background of MRC1 Recombinant Protein**
The macrophage mannose receptor 1 (MRC1. also known as CD206) is a transmembrane glycoprotein belonging to the C-type lectin family. It is primarily expressed on the surface of macrophages, dendritic cells, and specific endothelial cells, playing a pivotal role in innate immunity and homeostasis. MRC1 recognizes pathogen-associated molecular patterns (PAMPs) through its carbohydrate-recognition domains (CRDs), binding to terminal mannose, fucose, or N-acetylglucosamine residues on microbial surfaces. This interaction facilitates pathogen phagocytosis, antigen presentation, and immune response modulation.
Recombinant MRC1 protein is engineered in vitro using expression systems (e.g., mammalian, insect, or bacterial cells) to mimic the native protein’s functional domains. It typically includes the extracellular region, comprising multiple CRDs, a fibronectin type II domain, and a cysteine-rich domain, essential for ligand binding and receptor dimerization. Purification tags (e.g., His-tag or Fc-fusion) are often added for isolation and detection.
Research applications of MRC1 recombinant protein span immunology, infectious diseases, and cancer. It is used to study receptor-ligand interactions, immune evasion mechanisms of pathogens (e.g., Mycobacterium tuberculosis, HIV), and tumor-associated macrophage (TAM) behaviors in tumor microenvironments. Additionally, it serves as a tool for developing targeted therapies, such as drug delivery systems exploiting MRC1’s endocytic capacity.
Despite its utility, challenges remain in preserving post-translational modifications (e.g., glycosylation) during recombinant production, which can affect ligand binding. Ongoing studies aim to optimize expression systems to enhance functional fidelity, broadening its applicability in biomedical research and therapeutic innovation.
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