关键信息
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基因名
FBXO27
- 应用
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别名
FBXO27; FBG5; FBX27F-box only protein 27; F-box/G-domain protein 5
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种属
Human
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表达系统
E. coli
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标签
GST-tag at N-terminal
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纯度
Greater than 90% as determined by SDS-PAGE.
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蛋白编号
Q8NI29
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表达区间
1-283aa
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氨基酸序列
MGASVSRGRAARVPAPEPEPEEALDLSQLPPELLLVVLSHVPPRTLLGRCRQVCRGWRALVDGQALWLLILARDHGATGRALLHLARSCQSPARNARPCPLGRFCARRPIGRNLIRNPCGQEGLRKWMVQHGGDGWVVEENRTTVPGAPSQTCFVTSFSWCCKKQVLDLEEEGLWPELLDSGRIEICVSDWWGARHDSGCMYRLLVQLLDANQTVLDKFSAVPDPIPQWNNNACLHVTHVFSNIKMGVRFVSFEHRGQDTQFWAGHYGARVTNSSVIVRVRLS
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分子量
58 kDa
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内毒素
< 1.0 EU per μg protein as determined by the LAL method.
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性状
Freeze-dried powder
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缓冲液
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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复溶方法
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- 个性化定制
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稳定性测试
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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保存条件 & 期限
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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运输条件
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
质检流程
相关产品
背景信息
FBXO27 is a member of the F-box protein family, which plays a critical role in the ubiquitin-proteasome system, a key pathway for protein degradation and cellular regulation. Studies have shown that FBXO27 is involved in targeting specific substrates for ubiquitination, thereby influencing various cellular processes such as cell cycle regulation, apoptosis, and signal transduction. Recent research has highlighted its potential implications in cancer biology, particularly due to its association with the regulation of key tumor suppressors and oncogenes. Given its significant role in modulating cellular pathways, FBXO27 has garnered interest as a potential therapeutic target and biomarker in cancer diagnostics. To further understand the functional mechanisms of FBXO27, researchers have begun to focus on the production and characterization of recombinant FBXO27 protein. This approach allows for detailed biochemical and biophysical studies, facilitating insights into its interaction with substrates and other components of the ubiquitin-proteasome system. The exploration of FBXO27's structure-function relationship also paves the way for identifying small molecules that could modulate its activity, offering new avenues for targeted therapies in diseases associated with its dysregulation.
