关键信息
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基因名
FBXO5
- 应用
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别名
Early mitotic inhibitor 1; Emi 1; EMI1; F box only protein 5; F box protein 5; F box protein Fbx 5; F box protein Fbx5; F-box only protein 5; FBX5; FBX5_HUMAN; Fbxo31; fbxo5
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种属
Human
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表达系统
E. coli
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标签
GST-tag at N-terminal
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纯度
Greater than 90% as determined by SDS-PAGE.
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蛋白编号
Q9UKT4
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表达区间
1-447aa
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氨基酸序列
MSRRPCSCALRPPRCSCSASPSAVTAAGRPRPSDSCKEESSTLSVKMKCDFNCNHVHSGLKLVKPDDIGRLVSYTPAYLEGSCKDCIKDYERLSCIGSPIVSPRIVELETESKRLYNKENQHVQQTLNSTNEIEALETSRLYEDSGYSSFSLQSGLSEHEEGSLLEENFGDSLQSCLLQIQSPDQYPNKNLLPVLHFEKVVCSTLKKNAKRNPKVDREMLKEIIARGNFRLQNIIGRKMGLECVDILSELFRRGLRHVLATILAQLSDMDLINVSKVSTTWKEILEDDKGAFQLYSKAIQRVTENNNKFSPHASTREYVMFRTPLASVQKSAAQTSLKKDAQTKLSNQGDQKGSTYSRHNEFSEVAKTLKKNESLKACIRCNSPAKYDCYLQRATCKREGCGFDYCTKCLCNYHTTKDCSDGKLLKASCKIGPLPGTKKSKKNLRRL
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分子量
75.57 kDa
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内毒素
< 1.0 EU per μg protein as determined by the LAL method.
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性状
Freeze-dried powder
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缓冲液
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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复溶方法
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- 个性化定制
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稳定性测试
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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保存条件 & 期限
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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运输条件
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
质检流程
相关产品
背景信息
FBXO5, also known as F-box only protein 5, is a member of the F-box family of proteins, which play a crucial role in the ubiquitin-proteasome system by acting as substrates for the SCF (SKP1-CUL1-F-box protein) ubiquitin ligase complex. This complex is integral for regulating various cellular processes, including cell cycle progression, signal transduction, and transcription. FBXO5 is particularly important in the regulation of the G1–S phase transition of the cell cycle, where it mediates the degradation of specific cell cycle inhibitors. Abnormal expression of FBXO5 has been implicated in various cancers, making it a potential target for therapeutic intervention. Researchers are increasingly interested in the characterization of recombinant FBXO5 protein to elucidate its function, molecular interactions, and potential as a biomarker or therapeutic target in oncology. The production of recombinant FBXO5 allows for in-depth studies on its activity and interactions with other cellular proteins, paving the way for novel cancer treatments and furthering our understanding of cell cycle regulation and tumorigenesis.
