关键信息
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基因名
UBE2O
- 应用
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别名
E2 230K; EC=6.3.2.19; FLJ12878; KIAA1734; Likely Ortholog of Mouse Ubiquitin Conjugating Enzyme; RP23 193A16.5; UBE2O; UBE2O_HUMAN; Ubiquitin carrier Protein O; Ubiquitin conjugating enzyme E2 O; Ubiquitin conjugating enzyme E2 of 230 kDa; ubiquitin conjugating enzyme E2O; Ubiquitin Protein ligase O; Ubiquitin-conjugating enzyme E2 O; Ubiquitin-conjugating enzyme E2 of 230 kDa; Ubiquitin-conjugating enzyme E2-230K; Ubiquitin-Protein ligase O
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种属
Human
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表达系统
E. coli
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标签
GST-tag at N-terminal
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纯度
Greater than 90% as determined by SDS-PAGE.
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蛋白编号
Q9C0C9
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表达区间
1-741 aa
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氨基酸序列
MTSADVMWQDGSVECNIRSNDLFPVHHLDNNEFCPGDFVVDKRVQSCPDPAVYGVVQSGDHIGRTCMVKWFKLRPSGDDVELIGEEEDVSVYDIADHPDFRFRTTDIVIRIGNTEDGAPHKEDEPSVGQVARVDVSSKVEVVWADNSKTIILPQHLYNIESEIEESDYDSVEGSTSGASSDEWEDDSDSWETDNGLVEDEHPKIEEPPIPPLEQPVAPEDKGVVISEEAATAAVQGAVAMAAPMAGLMEKAGKDGPPKSFRELKEAIKILESLKNMTVEQLLTGSPTSPTVEPEKPTREKKFLDDIKKLQENLKKTLDNVAIVEEEKMEAVPDVERKEDKPEGQSPVKAEWPSETPVLCQQCGGKPGVTFTSAKGEVFSVLEFAPSNHSFKKIEFQPPEAKKFFSTVRKEMALLATSLPEGIMVKTFEDRMDLFSALIKGPTRTPYEDGLYLFDIQLPNIYPAVPPHFCYLSQCSGRLNPNLYDNGKVCVSLLGTWIGKGTERWTSKSSLLQVLISIQGLILVNEPYYNEAGFDSDRGLQEGYENSRCYNEMALIRVVQSMTQLVRRPPEVFEQEIRQHFSTGGWRLVNRIESWLETHALLEKAQALPNGVPKASSSPEPPAVAELSDSGQQEPEDGGPAPGEASQGSDSEGGAQGLASASRDHTDQTSETAPDASVPPSVKPKKRRKSYRSFLPEKSGYPDIGFPLFPLSKGFIKSIRGVLTQFRAALLEAGMPECTEDK
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分子量
108.1 kDa
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内毒素
< 1.0 EU per μg protein as determined by the LAL method.
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性状
Freeze-dried powder
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缓冲液
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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复溶方法
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- 个性化定制
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稳定性测试
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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保存条件 & 期限
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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运输条件
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
质检流程
相关产品
背景信息
UBE2O, a member of the ubiquitin-conjugating enzyme family, plays a critical role in the ubiquitin-proteasome system, which governs protein degradation and cellular homeostasis. Research has shown that UBE2O is involved in various cellular processes, including DNA repair, cell cycle regulation, and the modulation of immune responses. Dysregulation of UBE2O has been linked to several diseases, including cancer, where it can either promote tumor progression or act as a tumor suppressor depending on the context. The study of recombinant UBE2O protein has gained traction as it enables the detailed investigation of its enzymatic activity, substrate specificity, and interaction with other proteins in the ubiquitination pathway. Understanding UBE2O's functional mechanisms could illuminate its potential as a therapeutic target, as altering its activity may offer novel strategies for disease intervention. Furthermore, advancements in protein expression and purification techniques have made it feasible to produce large quantities of functional UBE2O for structural and biochemical studies, facilitating a deeper insight into its role in cellular regulation and disease. This growing body of research underscores the importance of UBE2O in cellular biology and its potential implications for developing targeted therapies in diseases characterized by altered protein degradation pathways.
