关键信息
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基因名
HDC
- 应用
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别名
HDC；Histidine decarboxylase
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种属
Human
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表达系统
E. coli
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标签
His tag N-Terminus
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纯度
Greater than 90% as determined by SDS-PAGE.
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蛋白编号
P19113
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表达区间
1-662aa
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氨基酸序列
MMEPEEYRERGREMVDYICQYLSTVRERRVTPDVQPGYLRAQLPESAPEDPDSWDSIFGDIERIIMPGVVHWQSPHMHAYYPALTSWPSLLGDMLADAINCLGFTWASSPACTELEMNVMDWLAKMLGLPEHFLHHHPSSQGGGVLQSTVSESTLIALLAARKNKILEMKTSEPDADESCLNARLVAYASDQAHSSVEKAGLISLVKMKFLPVDDNFSLRGEALQKAIEEDKQRGLVPVFVCATLGTTGVCAFDCLSELGPICAREGLWLHIDAAYAGTAFLCPEFRGFLKGIEYADSFTFNPSKWMMVHFDCTGFWVKDKYKLQQTFSVNPIYLRHANSGVATDFMHWQIPLSRRFRSVKLWFVIRSFGVKNLQAHVRHGTEMAKYFESLVRNDPSFEIPAKRHLGLVVFRLKGPNCLTENVLKEIAKAGRLFLIPATIQDKLIIRFTVTSQFTTRDDILRDWNLIRDAATLILSQHCTSQPSPRVGNLISQIRGARAWACGTSLQSVSGAGDDPVQARKIIKQPQRVGAGPMKRENGLHLETLLDPVDDCFSEEAPDATKHKLSSFLFSYLSVQTKKKTVRSLSCNSVPVSAQKPLPTEASVKNGGSSRVRIFSRFPEDMMMLKKSAFKKLIKFYSVPSFPECSSQCGLQLPCCPLQAMV
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分子量
76.2 kDa
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内毒素
< 1.0 EU per μg protein as determined by the LAL method.
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性状
Freeze-dried powder
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缓冲液
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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复溶方法
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- 个性化定制
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稳定性测试
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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保存条件 & 期限
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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运输条件
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
质检流程
相关产品
背景信息
The study of HDC (Histidine Decarboxylase) recombinant protein is grounded in its crucial role in the biosynthesis of histamine, a biogenic amine that serves various physiological functions, including the regulation of immune responses, neurotransmission, and gastric acid secretion. Histamine is synthesized from the amino acid histidine through the enzymatic action of HDC, making this enzyme a significant focus in understanding related disorders such as allergies, inflammatory diseases, and neuropsychiatric conditions. The production of recombinant HDC proteins allows researchers to investigate the enzyme's structure and function in detail, providing insights into its catalytic mechanisms and regulation. Moreover, recombinant HDC can be utilized in pharmacological studies, contributing to the development of novel therapies targeting histamine-related pathways. By producing purified HDC protein in host systems, scientists aim to facilitate high-throughput screening of potential inhibitors and modifiers, thereby enhancing our understanding of histamine's role in health and disease. The advances in recombinant DNA technology have made it possible to manipulate HDC genes, leading to the generation of mutant variants that can deepen our comprehension of histamine's diverse biological functions and its implications in various pathological states. Consequently, HDC recombinant protein research holds significant implications for both basic science and clinical applications, paving the way for innovative approaches to manage disorders associated with histamine dysregulation.
