关键信息
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基因名
FLOT2
- 应用
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别名
FLOT2；ESA1；M17S1；Flotillin-2
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种属
Human
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表达系统
E. coli
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标签
His tag N-Terminus
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纯度
Greater than 90% as determined by SDS-PAGE.
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蛋白编号
Q14254
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表达区间
50-428aa
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氨基酸序列
MTLQPRCEDVETAEGVALTVTGVAQVKIMTEKELLAVACEQFLGKNVQDI KNVVLQTLEGHLRSILGTLTVEQIYQDRDQFAKLVREVAAPDVGRMGIEI LSFTIKDVYDKVDYLSSLGKTQTAVVQRDADIGVAEAERDAGIREAECKK EMLDVKFMADTKIADSKRAFELQKSAFSEEVNIKTAEAQLAYELQGAREQ QKIRQEEIEIEVVQRKKQIAVEAQEILRTDKELIATVRRPAEAEAHRIQQ IAEGEKVKQVLLAQAEAEKIRKIGEAEAAVIEAMGKAEAERMKLKAEAYQ KYGDAAKMALVLEALPQIAAKIAAPLTKVDEIVVLSGDNSKVTSEVNRLL AELPASVHALTGVDLSKIPLIKKATGVQV
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分子量
67 kDa
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内毒素
< 1.0 EU per μg protein as determined by the LAL method.
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性状
Freeze-dried powder
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缓冲液
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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复溶方法
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- 个性化定制
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稳定性测试
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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保存条件 & 期限
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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运输条件
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
质检流程
相关产品
背景信息
FLOT2, or flotillin-2, is a membrane protein that plays a crucial role in the formation and function of lipid rafts, which are specialized microdomains in the cell membrane involved in various cellular processes, including signaling, endocytosis, and trafficking. Research into FLOT2 has gained increasing attention due to its association with several physiological and pathological conditions, such as cancer, neurodegenerative diseases, and infections. FLOT2 is known to facilitate the internalization of pathogens and signaling molecules by promoting the clustering of receptors and other proteins in lipid rafts. The characterization of FLOT2 as a potential biomarker and therapeutic target has driven the need for producing recombinant FLOT2 protein to study its function, interactions, and structural properties in detail. Recombinant protein expression systems allow for the generation of ample quantities of pure FLOT2, enabling researchers to investigate its role in various cellular contexts and explore its potential as a drug target. Furthermore, understanding the mechanisms underlying FLOT2-mediated processes can uncover novel strategies for therapeutic intervention in diseases where lipid raft dynamics are disrupted. Overall, the study of FLOT2 and its recombinant protein represents a significant avenue in cell biology and therapeutic development, with implications for improving disease outcomes and therapeutic precision.
