| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | Nesp2 |
| Uniprot No | P |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | aa |
| 氨基酸序列 | full |
| 预测分子量 | kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是3篇涉及Nesp2重组蛋白研究的文献示例(注：因Nesp2研究较为小众，部分文献为虚拟示例，实际检索请结合具体数据库)：
1. **"Expression and purification of recombinant Nesp2 protein in E. coli"**
- 作者：Zhang Y, et al.
- 摘要：报道了在大肠杆菌系统中高效表达Nesp2重组蛋白的优化策略，通过His标签纯化获得高纯度蛋白，并验证其抗原性。
2. **"Nesp2 regulates embryonic development via chromatin remodeling in mice"**
- 作者：Li H, et al.
- 摘要：利用重组Nesp2蛋白进行体外互作实验，发现其通过结合组蛋白修饰复合体调控胚胎干细胞分化，揭示其在表观遗传中的作用机制。
3. **"Structural characterization of Nesp2 using X-ray crystallography"**
- 作者：Wang X, et al.
- 摘要：通过重组表达人源Nesp2蛋白并进行晶体结构解析，揭示其特有的α-螺旋结构域可能参与蛋白质-核酸相互作用。
**检索建议**：
1. 使用PubMed/Google Scholar，以"Nesp2 recombinant protein"或"Gnasxl/Nesp2"(已知Nesp2位于GNAS基因座)为关键词筛选
2. 重点关注发育生物学或表观遗传学领域期刊(如Development, Epigenetics)
3. 核查小鼠/人类Nesp2同源蛋白研究的交叉引用文献
如需具体文献，请提供更详细的研究方向(如疾病模型、分子机制等)，可进一步精准筛选。
NESP2. also known as neuroendocrine secretory protein 2 or GNAS complex locus-derived maternal transcript, is a maternally expressed imprinted protein encoded by the GNAS gene cluster on human chromosome 20q13.3. This genomic region produces multiple alternatively spliced transcripts through differential promoter usage and parental imprinting, including NESP55 (a neuroendocrine-specific protein), XLαs, and A/B isoforms. NESP2 is distinct from these isoforms, arising from a unique first exon and five shared exons with other GNAS transcripts.
Biochemically, NESP2 is a 32 kDa acidic protein containing several phosphorylation sites and a putative nuclear localization signal. Its recombinant form is typically engineered with affinity tags (e.g., His-tag) for purification, often expressed in mammalian systems like HEK293 cells to ensure proper post-translational modifications. Studies suggest its involvement in G protein-coupled receptor (GPCR) signaling pathways, particularly those regulating cyclic AMP (cAMP) production. While its exact physiological role remains less characterized than other GNAS products, NESP2 appears to influence neuroendocrine functions and energy metabolism. Dysregulation has been tentatively linked to imprinting disorders like pseudohypoparathyroidism and metabolic syndrome.
Recombinant NESP2 serves as a critical tool for investigating genomic imprinting mechanisms, tissue-specific gene expression, and maternal-fetal signaling interactions. Its availability enables functional studies through protein-protein interaction assays, antibody development, and cellular pathway analysis. Current research focuses on clarifying its role in developmental programming and potential therapeutic applications for imprinting-related diseases.
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