关键信息
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基因名
GIT2
- 应用
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别名
ARF GAP GIT2; ARF GTPase activating protein GIT2; ARF GTPase-activating protein GIT2; CAT 2; CAT-2; CAT2; Cool associated tyrosine phosphorylated protein 2; Cool interacting tyrosine phosphorylated protein 2; Cool-interacting tyrosine-phosphorylated protein 2; DKFZp686G01261; G protein coupled receptor kinase interacting ArfGAP 2
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种属
Human
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表达系统
E. coli
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标签
GST-tag at N-terminal
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纯度
Greater than 90% as determined by SDS-PAGE.
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蛋白编号
Q14161
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表达区间
1-471aa
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氨基酸序列
MSKRLRSSEVCADCSGPDPSWASVNRGTFLCDECCSVHRSLGRHISQVRHLKHTPWPPTLLQMVETLYNNGANSIWEHSLLDPASIMSGRRKANPQDKVHPNKAEFIRAKYQMLAFVHRLPCRDDDSVTAKDLSKQLHSSVRTGNLETCLRLLSLGAQANFFHPEKGNTPLHVASKAGQILQAELLAVYGADPGTQDSSGKTPVDYARQGGHHELAERLVEIQYELTDRLAFYLCGRKPDHKNGQHFIIPQMADSSLDLSELAKAAKKKLQSLSNHLFEELAMDMYDEVDRRETDAVWLATQNHSALVTETTVVPFLPVNPEYSSTRNQGRQKLARFNAHEFATLVIDILSDAKRRQQGSSLSGSKDNVELILKTINNQHSVESQDNDQPDYDSVASDEDTDLETTASKTNRQKSLDSDLSDGPVTVQEFMEVKNALVASEAKIQQLMKVNNNLSDELRIMQKKLLGKDAN
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分子量
77.33 kDa
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内毒素
< 1.0 EU per μg protein as determined by the LAL method.
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性状
Freeze-dried powder
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缓冲液
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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复溶方法
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- 个性化定制
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稳定性测试
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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保存条件 & 期限
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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运输条件
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
质检流程
相关产品
背景信息
GIT2 (GTPase-activating protein for Rho family GTPases 2) is a member of the Git family of proteins, which play crucial roles in various cellular processes, including cell migration, adhesion, and signal transduction. Research into GIT2 has gained significant attention due to its involvement in the regulation of Rho GTPases, which are critical for cytoskeletal dynamics and cellular morphology. Dysregulation of GIT2 has been linked to several diseases, including cancer, where altered cell migration and invasion contribute to tumor progression and metastasis. Additionally, GIT2 interacts with various signaling pathways, making it a potential therapeutic target for interventions in related pathologies. Studies exploring GIT2's structural characteristics and functional domains have highlighted its significance in cellular signaling networks. Moreover, the development of GIT2 recombinant proteins allows for advanced investigations into its biochemical properties and interactions with other cellular components. Understanding GIT2 at the molecular level could provide insights into its role in health and disease, paving the way for the development of novel therapeutic strategies aimed at modulating its function in pathological conditions.
