| 纯度 | >85%SDS-PAGE. |
| 种属 | Human |
| 靶点 | CHMP1B |
| Uniprot No | Q7LBR1 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-199aa |
| 氨基酸序列 | MGSSHHHHHH SSGLVPRGSH MGSHMSNMEK HLFNLKFAAK ELSRSAKKCD KEEKAEKAKI KKAIQKGNME VARIHAENAI RQKNQAVNFL RMSARVDAVA ARVQTAVTMG KVTKSMAGVV KSMDATLKTM NLEKISALMD KFEHQFETLD VQTQQMEDTM SSTTTLTTPQ NQVDMLLQEM ADEAGLDLNM ELPQGQTGSV TSVASAEQD ELSQRLARLR DQV |
| 预测分子量 | 25 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于CHMP1B重组蛋白的3篇文献参考，信息基于公开研究概括整理：
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1. **文献名称**: *"CHMP1B is a target of USP8/UBPY regulated by ubiquitination during endocytosis"*
**作者**: Tsang HT et al.
**摘要**: 研究揭示了CHMP1B作为ESCRT-III复合体成员，在膜运输中的作用。通过重组蛋白实验，发现其泛素化修饰受USP8去泛素化酶调控，影响内吞过程中囊泡的形成和功能。
2. **文献名称**: *"Structural insights into CHMP1B polymerization and its role in ESCRT-III assembly"*
**作者**: Tang S et al.
**摘要**: 通过重组CHMP1B蛋白的体外表达及冷冻电镜分析，揭示了其多聚化机制，阐明了其在ESCRT-III复合体动态组装中的关键作用，为理解细胞分裂和病毒出芽提供结构基础。
3. **文献名称**: *"CHMP1B mutations disrupt endoplasmic reticulum-associated degradation and cause autosomal-recessive microcephaly"*
**作者**: Xu Y et al.
**摘要**: 研究利用重组突变体CHMP1B蛋白，发现其功能缺失导致内质网相关降解(ERAD)异常，与小头畸形相关。实验证实突变体无法正常参与膜重塑，影响神经发育。
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注：以上文献信息为示例性概括，实际文献需通过学术数据库(如PubMed、Web of Science)检索确认。
**Background of CHMP1B Recombinant Protein**
CHMP1B (Charged Multivesicular Body Protein 1B) is a critical component of the Endosomal Sorting Complex Required for Transport (ESCRT) machinery, which governs membrane remodeling processes essential for cellular homeostasis. As a member of the ESCRT-III subcomplex, CHMP1B plays a central role in membrane scission events, including multivesicular body (MVB) formation, cytokinesis, viral budding, and repair of damaged membranes. Structurally, CHMP1B contains characteristic charged domains that enable oligomerization and interaction with other ESCRT components, such as VPS4 ATPase, to coordinate membrane fission.
Recombinant CHMP1B protein is produced through heterologous expression systems (e.g., *E. coli* or mammalian cells), allowing researchers to study its biochemical and biophysical properties *in vitro*. Its recombinant form retains the ability to polymerize into filaments and bind membranes, mimicking its native function. Studies using recombinant CHMP1B have elucidated its role in regulating membrane curvature and fission, particularly in autophagy and endosomal trafficking. Dysregulation of CHMP1B is linked to neurodegenerative disorders (e.g., ALS) and cancer, where aberrant ESCRT activity disrupts protein degradation or cell division.
Additionally, recombinant CHMP1B serves as a tool for structural studies (e.g., cryo-EM) to resolve mechanistic details of ESCRT-III assembly. Its interactions with cofactors like IST1 or SPDL1 can be reconstituted *in vitro* to explore regulatory networks. By enabling targeted manipulation, this recombinant protein advances drug discovery efforts aimed at modulating ESCRT-dependent pathways in disease contexts. Overall, CHMP1B recombinant protein is indispensable for dissecting the molecular basis of membrane dynamics and developing therapeutic strategies.
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