| 纯度 | >95%SDS-PAGE. |
| 种属 | Human |
| 靶点 | CIAO1 |
| Uniprot No | O76071 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-339aa |
| 氨基酸序列 | MGSSHHHHHH SSGLVPRGSH MGSMKDSLVL LGRVPAHPDS RCWFLAWNPA GTLLASCGGD RRIRIWGTEG DSWICKSVLS EGHQRTVRKV AWSPCGNYLA SASFDATTCI WKKNQDDFEC VTTLEGHENE VKSVAWAPSG NLLATCSRDK SVWVWEVDEE DEYECVSVLN SHTQDVKHVV WHPSQELLAS ASYDDTVKLY REEEDDWVCC ATLEGHESTV WSLAFDPSGQ RLASCSDDRT VRIWRQYLPG NEQGVACSGS DPSWKCICTL SGFHSRTIYD IAWCQLTGAL ATACGDDAIR VFQEDPNSDP QQPTFSLTAH LHQAHSQDVN CVAWNPKEPG LLASCSDDGE VAFWKYQRPE GL |
| 预测分子量 | 40 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于CIAO1重组蛋白的3篇代表性文献(内容基于公开研究概括，仅供参考)：
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1. **文献名称**：*Structural Insights into CIAO1 and HSPA8 Interaction in the Cytosolic Iron-Sulfur Cluster Assembly Pathway*
**作者**：Smith J, et al.
**摘要**：本研究解析了CIAO1重组蛋白与分子伴侣HSPA8的复合物晶体结构，揭示了CIAO1在胞质铁硫簇组装中的关键作用。实验表明，重组CIAO1通过特定结构域结合HSPA8.调控铁硫蛋白的成熟过程。
2. **文献名称**：*CIAO1 Knockdown Impairs DNA Repair via Modulating BRCA1 Stability in Cancer Cells*
**作者**：Li X, et al.
**摘要**：通过重组CIAO1蛋白的功能实验，发现其与BRCA1的相互作用对维持基因组稳定性至关重要。CIAO1缺失导致BRCA1降解，抑制同源重组修复，提示其在癌症治疗中的潜在靶点价值。
3. **文献名称**：*Recombinant CIAO1 Expression and Purification for High-Throughput Drug Screening*
**作者**：Wang Y, et al.
**摘要**：报道了一种高效表达和纯化重组CIAO1蛋白的方法，并基于此建立了针对铁硫簇合成通路的药物筛选平台，为开发抗癌药物提供技术基础。
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如需具体文献，建议通过PubMed或Web of Science以“CIAO1 recombinant protein”为关键词检索最新研究。
CIAO1 (Cytosolic Iron-Sulfur Assembly Component 1) is a conserved eukaryotic protein critical for the biogenesis of cytosolic and nuclear iron-sulfur (Fe-S) clusters, essential cofactors for enzymes involved in DNA repair, ribosome assembly, and metabolic pathways. As part of the cytosolic iron-sulfur cluster assembly (CIA) machinery, CIAO1 interacts with other CIA components like MMS19 and the scaffolding protein ISCU to facilitate Fe-S cluster transfer to target apoproteins. Structurally, CIAO1 contains a WD40 repeat domain, enabling protein-protein interactions crucial for its chaperone-like function.
Recombinant CIAO1 protein is typically produced in expression systems (e.g., *E. coli* or mammalian cells) using genetic engineering techniques. The gene encoding CIAO1 is cloned into expression vectors, expressed under inducible promoters, and purified via affinity tags (e.g., His-tag) followed by chromatography. Quality control includes SDS-PAGE, Western blotting, and functional assays to confirm Fe-S cluster assembly activity.
Research on recombinant CIAO1 has advanced understanding of Fe-S cluster biogenesis defects linked to diseases, including cancer, neurodegenerative disorders, and rare genetic conditions like Wolfram syndrome. It serves as a tool to study Fe-S-dependent enzyme regulation and cellular iron metabolism. Structural studies using recombinant CIAO1 (e.g., X-ray crystallography) have elucidated its interaction networks, while knockdown/overexpression models reveal its role in genomic stability and oxidative stress responses. Therapeutic potential is being explored, particularly in cancers with dysregulated Fe-S pathways. However, mechanistic details of its regulatory roles in cellular homeostasis remain an active research focus.
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