| 纯度 | >85%SDS-PAGE. |
| 种属 | Human |
| 靶点 | CLTB |
| Uniprot No | P09497 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-211aa |
| 氨基酸序列 | MGSSHHHHHH SSGLVPRGSH MGSMADDFGF FSSSESGAPE AAEEDPAAAF LAQQESEIAG IENDEGFGAP AGSHAAPAQP GPTSGAGSED MGTTVNGDVF QEANGPADGY AAIAQADRLT QEPESIRKWR EEQRKRLQEL DAASKVTEQE WREKAKKDLE EWNQRQSEQV EKNKINNRAS EEAFVKESKE ETPGTEWEKV AQLCDFNPKS SKQCKDVSRL RSVLMSLKQT PLSR |
| 预测分子量 | 26 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于CLTB(Clathrin Light Chain B)重组蛋白研究的假设性参考文献示例(注：以下内容为模拟示例，非真实文献)：
1. **文献名称**: "Expression and Purification of Recombinant CLTB in E. coli for Functional Studies"
**作者**: Zhang, L. et al.
**摘要**: 本研究报道了人源CLTB基因在大肠杆菌系统中的高效表达及纯化方法。通过优化密码子及诱导条件，成功获得可溶性重组蛋白，并利用镍柱亲和层析纯化。Western blot验证表明蛋白具有免疫活性，为后续研究CLTB在网格蛋白组装中的作用提供了材料基础。
2. **文献名称**: "Structural Analysis of CLTB in Clathrin-Mediated Endocytosis"
**作者**: Müller, R. & Tanaka, K.
**摘要**: 作者通过重组CLTB蛋白的晶体学研究，揭示了其与网格蛋白重链(CHC)的相互作用界面。实验表明CLTB通过特定α螺旋结构域调控网格蛋白三腿复合物的稳定性，为理解细胞内吞过程的分子机制提供了结构生物学依据。
3. **文献名称**: "Functional Characterization of CLTB Isoforms in Vesicle Trafficking"
**作者**: Gupta, S. & Lee, J.Y.
**摘要**: 通过构建CLTB两种剪接变体的重组蛋白，本研究比较了它们在COX细胞中对囊泡运输效率的影响。荧光标记实验显示CLTB-V2相比V1具有更强的AP2复合物结合能力，提示其剪接变异可能参与运输通路的特异性调控。
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**检索建议**：
如需真实文献，建议在PubMed或Google Scholar使用以下关键词组合检索：
`"CLTB recombinant protein expression"`
`"Clathrin Light Chain B purification"`
`"CLTB structure-function analysis"`
可筛选近5-10年文献以获取最新研究方法。
**Background of CLTB Recombinant Protein**
Clathrin light chain B (CLTB) is a critical component of clathrin, a multifunctional protein complex essential for intracellular vesicle formation and membrane trafficking. Clathrin consists of three heavy chains (CLTC) and three light chains, which include two isoforms—CLTA (light chain A) and CLTB (light chain B). These light chains regulate clathrin’s assembly, disassembly, and interactions with other proteins during endocytosis and organelle biogenesis. CLTB, encoded by the *CLTB* gene in humans, plays a role in modulating clathrin heavy chain flexibility and binding to accessory proteins, thereby influencing vesicle coat stability and cargo sorting.
Recombinant CLTB protein is produced using genetic engineering techniques, typically expressed in *E. coli* or mammalian systems, followed by purification to ensure high specificity and functionality. This engineered protein retains the native structure and biochemical properties of endogenous CLTB, enabling researchers to study its interactions, structural dynamics, and regulatory mechanisms in vitro.
CLTB’s involvement in clathrin-mediated trafficking links it to critical cellular processes, including neurotransmitter release, receptor internalization, and nutrient uptake. Dysregulation of CLTB has been implicated in neurological disorders, cancer, and viral entry mechanisms, making it a target for therapeutic research. Recombinant CLTB serves as a valuable tool for investigating these pathways, screening drug candidates, or developing assays to probe clathrin-dependent cellular functions.
Overall, CLTB recombinant protein bridges fundamental research and applied studies, offering insights into intracellular transport biology and potential clinical applications in disease diagnosis or treatment.
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