| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | COTL1 |
| Uniprot No | Q14019 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-142aa |
| 氨基酸序列 | MGSSHHHHHH SSGLVPRGSH MATKIDKEAC RAAYNLVRDD GSAVIWVTFK YDGSTIVPGE QGAEYQHFIQ QCTDDVRLFA FVRFTTGDAMSKRSKFALIT WIGENVSGLQ RAKTGTDKTL VKEVVQNFAK EFVISDRKEL EEDFIKSELK KAGGANYDAQ TE |
| 预测分子量 | 18 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于COTL1重组蛋白的模拟参考文献示例(注：文献信息为示例性质，非真实存在)：
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1. **《Recombinant COTL1 enhances macrophage migration via actin polymerization》**
- **作者**: Smith A, et al.
- **摘要**: 本研究利用重组COTL1蛋白探究其在巨噬细胞迁移中的作用，发现其通过促进肌动蛋白聚合，增强细胞伪足形成和趋化因子诱导的迁移能力，提示其在炎症反应中的调控机制。
2. **《COTL1 interacts with 5-lipoxygenase and modulates leukotriene biosynthesis》**
- **作者**: Lee B, et al.
- **摘要**: 通过重组COTL1蛋白体外实验，揭示了其与5-脂氧合酶(5-LO)的直接结合，调节炎症介质白三烯的合成，为靶向COTL1的抗炎治疗提供了理论依据。
3. **《Recombinant COTL1 promotes cancer cell invasion by activating ERK signaling》**
- **作者**: Zhang C, et al.
- **摘要**: 研究发现重组COTL1蛋白在肿瘤微环境中通过激活ERK信号通路，上调基质金属蛋白酶(MMPs)表达，从而促进肿瘤细胞的侵袭和转移。
4. **《Expression and purification of functional COTL1 recombinant protein in E. coli》**
- **作者**: Wang D, et al.
- **摘要**: 报道了一种高效的大肠杆菌表达系统，用于生产可溶性重组COTL1蛋白，并通过体外结合实验验证其与F-actin的结合活性，为后续功能研究提供可靠工具。
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注：以上文献为示例性内容，实际研究中建议通过PubMed、Web of Science等平台检索真实文献。
Coactosin-like protein 1 (COTL1) is a conserved actin-binding protein involved in regulating cytoskeletal dynamics and inflammatory responses. It belongs to the ADF/cofilin family but lacks canonical actin-depolymerizing activity. Instead, COTL1 stabilizes F-actin filaments through its coactosin-like domain, facilitating cellular processes like migration and membrane protrusion. Structurally, human COTL1 contains ~140 amino acids with a central coiled-coil region critical for protein-protein interactions.
Recombinant COTL1 proteins are typically produced using bacterial (e.g., E. coli) or eukaryotic expression systems, enabling studies of its biochemical properties and interactions. Research shows COTL1 binds 5-lipoxygenase (5-LO), a key enzyme in leukotriene biosynthesis, suggesting its role in inflammatory pathways. This interaction positions COTL1 as a potential modulator of immune responses and allergic disorders.
In cancer biology, elevated COTL1 expression correlates with tumor progression and metastasis in various cancers, potentially through cytoskeleton remodeling and enhanced cell motility. Recent studies also implicate COTL1 in immune regulation, particularly in macrophage polarization and T-cell activation, highlighting its dual roles in both structural biology and signaling pathways.
The development of recombinant COTL1 has facilitated drug discovery efforts targeting inflammatory diseases and cancer. It serves as a tool for identifying small-molecule inhibitors or studying post-translational modifications that regulate its functions. However, its pleiotropic effects across different cell types necessitate careful evaluation in therapeutic contexts. Ongoing research continues to unravel its mechanistic complexity, positioning COTL1 as a multifunctional protein bridging cytoskeletal organization and disease pathology.
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