| 纯度 | >85%SDS-PAGE. |
| 种属 | Human |
| 靶点 | CPSF4 |
| Uniprot No | O95639 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-244aa |
| 氨基酸序列 | MQEIIASVDHIKFDLEIAVEQQLGAQPLPFPGMDKSGAAVCEFFLKAACGKGGMCPFRHISGEKTVVCKHWLRGLCKKGDQCEFLHEYDMTKMPECYFYSKFGECSNKECPFLHIDPESKIKDCPWYDRGFCKHGPLCRHRHTRRVICVNYLVGFCPEGPSCKFMHPRFELPMGTTEQPPLPQQTQPPAKQRTPQVIGVMQSQNSSAGNRGPRPLEQVTCYKCGEKGHYANRCTKGHLAFLSGQ |
| 预测分子量 | 54.5kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于CPSF4重组蛋白的参考文献示例(注：内容为示例性概括，具体文献可能需要根据实际研究调整)：
1. **文献名称**：*CPSF4 regulates viral RNA processing and promotes influenza A virus replication*
**作者**：Smith A, et al.
**摘要**：研究通过重组CPSF4蛋白揭示了其在甲型流感病毒RNA 3'末端加工中的作用，证明其与病毒聚合酶相互作用，调控病毒mRNA的切割和多聚腺苷酸化，影响病毒复制效率。
2. **文献名称**：*Structural insights into CPSF4 recognition of RNA motifs*
**作者**：Chen L, et al.
**摘要**：利用重组CPSF4蛋白进行X射线晶体学分析，解析了其与RNA结合的分子机制，发现其锌指结构域特异性识别AAUAAA信号序列，为mRNA成熟机制提供结构基础。
3. **文献名称**：*CPSF4 knockdown inhibits prostate cancer progression via alternative polyadenylation*
**作者**：Wang Y, et al.
**摘要**：通过表达重组CPSF4蛋白进行功能挽救实验，证实CPSF4通过调控致癌基因mRNA的3'末端加工，促进前列腺癌细胞增殖和转移。
4. **文献名称**：*Recombinant CPSF4 interacts with HIV-1 Rev to modulate viral RNA export*
**作者**：Gomez M, et al.
**摘要**：研究发现重组CPSF4蛋白与HIV-1 Rev蛋白直接结合，协同调控病毒RNA的核输出，揭示其在宿主-病毒相互作用中的新功能。
(注：以上文献为示例，实际引用需根据具体研究检索数据库如PubMed或Web of Science获取。)
CPSF4 (Cleavage and Polyadenylation Specificity Factor Subunit 4) is a critical component of the mRNA 3ʹ-end processing machinery in eukaryotes. It functions as part of the CPSF complex, which recognizes the polyadenylation signal (AAUAAA) in precursor mRNAs (pre-mRNAs) and coordinates cleavage and polyadenylation during transcription termination. This process ensures proper maturation of mRNA, influencing stability, nuclear export, and translation efficiency. CPSF4. also known as CPSF30. is a 30 kDa protein containing conserved zinc finger domains that mediate RNA binding and protein-protein interactions. Its role extends beyond canonical mRNA processing; studies suggest involvement in alternative polyadenylation, which regulates gene expression diversity, and in viral RNA processing for certain viruses.
Recombinant CPSF4 protein is engineered for in vitro studies to dissect molecular mechanisms of mRNA processing, screen for inhibitors, or study viral-host interactions. It is typically produced using bacterial (e.g., *E. coli*) or mammalian expression systems, often fused with tags like His-tag or GST for purification. Structural and functional analyses of recombinant CPSF4 have revealed insights into its RNA-binding specificity and interactions with other CPSF subunits (e.g., CPSF160. WDR33) or viral proteins.
Research on CPSF4 has implications in understanding diseases linked to dysregulated mRNA metabolism, including cancers and viral infections. For example, CPSF4 overexpression is associated with tumor progression, while its interaction with HIV-1 RNA highlights a potential antiviral target. Challenges remain in elucidating its post-translational modifications and context-dependent roles. Recombinant CPSF4 tools continue to advance both basic and applied research in gene expression regulation.
×
