| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | CSTF1 |
| Uniprot No | Q05048 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-431aa |
| 氨基酸序列 | MYRTKVGLKD RQQLYKLIIS QLLYDGYISI ANGLINEIKP QSVCAPSEQL LHLIKLGMEN DDTAVQYAIG RSDTVAPGTG IDLEFDADVQ TMSPEASEYE TCYVTSHKGP CRVATYSRDG QLIATGSADA SIKILDTERM LAKSAMPIEV MMNETAQQNM ENHPVIRTLY DHVDEVTCLA FHPTEQILAS GSRDYTLKLF DYSKPSAKRA FKYIQEAEML RSISFHPSGD FILVGTQHPT LRLYDINTFQ CFVSCNPQDQ HTDAICSVNY NSSANMYVTG SKDGCIKLWD GVSNRCITTF EKAHDGAEVC SAIFSKNSKY ILSSGKDSVA KLWEISTGRT LVRYTGAGLS GRQVHRTQAV FNHTEDYVLL PDERTISLCC WDSRTAERRN LLSLGHNNIV RCIVHSPTNP GFMTCSDDFR ARFWYRRSTT DVEHHHHHH |
| 预测分子量 | 49 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于CSTF1重组蛋白的3篇代表性文献的简要概括(文献信息为示例性描述，仅供参考)：
1. **文献名称**: *Structural insights into CSTF1-mediated mRNA 3' processing*
**作者**: Takagaki Y., et al.
**摘要**: 本研究通过重组表达并纯化人源CSTF1蛋白，结合X射线晶体学解析其三维结构，揭示了CSTF1与RNA结合的关键结构域及参与多聚腺苷酸化的分子机制，为mRNA加工机制提供了结构基础。
2. **文献名称**: *Functional characterization of recombinant CSTF1 in alternative polyadenylation*
**作者**: Mandel C.R., et al.
**摘要**: 作者利用大肠杆菌表达系统获得重组CSTF1蛋白，通过体外RNA结合实验和截短突变分析，证明CSTF1的N端结构域对识别特定poly(A)信号位点及调控选择性多聚腺苷酸化具有决定性作用。
3. **文献名称**: *Recombinant CSTF1 expression and its role in viral RNA processing*
**作者**: Shi Y., et al.
**摘要**: 该研究在昆虫细胞中重组表达并纯化CSTF1蛋白，发现其与某些RNA病毒(如疱疹病毒)的mRNA加工存在相互作用，提示CSTF1可能参与病毒RNA的宿主劫持过程，为抗病毒靶点研究提供线索。
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**注**：以上文献信息为模拟示例，实际文献需通过PubMed、Google Scholar等平台检索确认。建议使用关键词 "CSTF1 recombinant protein" 或 "CSTF1 cleavage stimulation factor" 结合具体研究领域(如结构、功能、疾病关联)筛选近年文献。
**Background of CSTF1 Recombinant Protein**
CSTF1 (Cleavage Stimulation Factor Subunit 1) is a critical component of the cleavage stimulation factor (CSTF) complex, which plays an essential role in mRNA 3ʹ-end processing. This process involves the cleavage and polyadenylation of nascent pre-mRNA transcripts, a fundamental step in eukaryotic gene expression. The CSTF complex, composed of CSTF1. CSTF2. and CSTF3 subunits, interacts with other processing factors to recognize polyadenylation signals, ensuring proper mRNA maturation and stability. CSTF1 specifically contributes to stabilizing the complex and enhancing its binding to RNA, thereby facilitating efficient cleavage.
Recombinant CSTF1 protein is produced using heterologous expression systems (e.g., *E. coli* or mammalian cells*) to enable functional and structural studies. Its recombinant form retains the ability to assemble into the CSTF complex, making it a valuable tool for investigating mRNA processing mechanisms, including how dysregulation contributes to diseases like cancer or neurological disorders. Researchers also utilize CSTF1 recombinant protein to study mutations or post-translational modifications that alter 3ʹ-end processing, potentially leading to aberrant mRNA stability or translation.
Additionally, CSTF1 has been implicated in alternative polyadenylation (APA), a process linked to mRNA diversity and cellular adaptation. Recombinant CSTF1 aids in dissecting APA's role in development, stress responses, and disease progression. Its applications extend to drug discovery, where modulating mRNA processing could offer therapeutic avenues. Overall, CSTF1 recombinant protein serves as a pivotal resource for advancing molecular biology, genomics, and biomedicine.
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