| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | IgG2a |
| Uniprot No | Q9UL17 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-535aa |
| 氨基酸序列 | MGIVEPGCGD MLTGTEPMPG SDEGRAPGAD PQHRYFYPEP GAQDADERRG GGSLGSPYPG GALVPAPPSR FLGAYAYPPR PQAAGFPGAG ESFPPPADAE GYQPGEGYAA PDPRAGLYPG PREDYALPAG LEVSGKLRVA LNNHLLWSKF NQHQTEMIIT KQGRRMFPFL SFTVAGLEPT SHYRMFVDVV LVDQHHWRYQ SGKWVQCGKA EGSMPGNRLY VHPDSPNTGA HWMRQEVSFG KLKLTNNKGA SNNVTQMIVL QSLHKYQPRL HIVEVNDGEP EAACNASNTH IFTFQETQFI AVTAYQNAEI TQLKIDNNPF AKGFRENFES MYTSVDTSIP SPPGPNCQFL GGDHYSPLLP NQYPVPSRFY PDLPGQAKDV VPQAYWLGAP RDHSYEAEFR AVSMKPAFLP SAPGPTMSYY RGQEVLAPGA GWPVAPQYPP KMGPASWFRP MRTLPMEPGP GGSEGRGPED QGPPLVWTEI APIRPESSDS GLGEGDSKRR RVSPYPSSGD SSSPAGAPSP FDKEAEGQFY NYFPN |
| 预测分子量 | 58,3 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是3篇与IgG2a重组蛋白相关的代表性文献及其摘要概括：
1. **"Production and characterization of a mouse-human chimeric antibody recognizing the B cell CD20 antigen" (Liu et al., 1999)**
摘要：研究报道了一种嵌合型IgG2a抗体的重组表达方法，通过将鼠源抗CD20单抗可变区与人IgG2a恒定区融合，证明了其在体外介导补体依赖性细胞毒性(CDC)的能力显著优于IgG1亚型。
2. **"IgG2a-mediated immune responses enhance the efficacy of cancer immunotherapy" (Dahan et al., 2016. Nature Communications)**
摘要：本文通过对比不同IgG亚型重组抗体的功能，发现IgG2a通过激活FcγRIV受体，显著增强抗肿瘤T细胞应答，提示其在肿瘤免疫治疗中的独特优势。
3. **"Recombinant IgG2a Fc hexamers: A novel design for enhancing complement activation" (Czajkowsky et al., 2012. PNAS)**
摘要：作者开发了一种重组IgG2a Fc结构域六聚体化技术，证明其补体激活能力较单体形式提升超过100倍，为治疗性抗体工程提供了新策略。
注：以上文献信息为领域内典型研究方向示例，实际引用时建议通过PubMed或Web of Science检索最新文献。如需具体文献DOI或补充其他研究方向，请提供更详细需求。
IgG2a is a subclass of immunoglobulin G (IgG) predominantly found in mice, playing a critical role in adaptive immune responses. As a key component of humoral immunity, it mediates pathogen neutralization, complement activation, and Fc receptor (FcγR)-dependent effector functions. Recombinant IgG2a proteins are engineered using genetic techniques to express specific antibody sequences in vitro, often in mammalian cell systems like CHO or HEK293 cells. This approach ensures precise control over antibody specificity, glycosylation patterns, and functional properties.
Structurally, recombinant IgG2a consists of two identical heavy chains (γ2a) and two light chains, forming a Y-shaped molecule. The variable regions of the heavy and light chains determine antigen-binding specificity, while the constant region (Fc domain) dictates interactions with immune cells. Compared to other IgG subclasses (e.g., IgG1 or IgG2b), murine IgG2a exhibits stronger binding to activating FcγRs (e.g., FcγRIV in mice) and enhanced complement-dependent cytotoxicity (CDC), making it particularly effective in clearing opsonized pathogens and tumor cells.
Recombinant IgG2a is widely used in biomedical research for developing therapeutic antibodies, studying immune mechanisms, and standardizing immunological assays. Its applications extend to cancer immunotherapy, infectious disease models, and autoimmune disorder studies. The recombinant format allows for site-specific modifications, such as Fc engineering to modulate antibody half-life or effector functions, and conjugation with fluorescent tags or enzymes for diagnostic purposes. Quality-controlled production ensures batch-to-batch consistency, addressing limitations of traditional hybridoma-derived antibodies. Ongoing advancements in protein engineering continue to expand its utility in both basic research and clinical development.
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