| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | DAAM1 |
| Uniprot No | Q9Y4D1 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-1078aa |
| 氨基酸序列 | MAPRKRGGRGISFIFCCFRNNDHPEITYRLRNDSNFALQTMEPALPMPPVEELDVMFSELVDELDLTDKHREAMFALPAEKKWQIYCSKKKDQEENKGATSWPEFYIDQLNSMAARKSLLALEKEEEEERSKTIESLKTALRTKPMRFVTRFIDLDGLSCILNFLKTMDYETSESRIHTSLIGCIKALMNNSQGRAHVLAHSESINVIAQSLSTENIKTKVAVLEILGAVCLVPGGHKKVLQAMLHYQKYASERTRFQTLINDLDKSTGRYRDEVSLKTAIMSFINAVLSQGAGVESLDFRLHLRYEFLMLGIQPVIDKLREHENSTLDRHLDFFEMLRNEDELEFAKRFELVHIDTKSATQMFELTRKRLTHSEAYPHFMSILHHCLQMPYKRSGNTVQYWLLLDRIIQQIVIQNDKGQDPDSTPLENFNIKNVVRMLVNENEVKQWKEQAEKMRKEHNELQQKLEKKERECDAKTQEKEEMMQTLNKMKEKLEKETTEHKQVKQQVADLTAQLHELSRRAVCASIPGGPSPGAPGGPFPSSVPGSLLPPPPPPPLPGGMLPPPPPPLPPGGPPPPPGPPPLGAIMPPPGAPMGLALKKKSIPQPTNALKSFNWSKLPENKLEGTVWTEIDDTKVFKILDLEDLERTFSAYQRQQDFFVNSNSKQKEADAIDDTLSSKLKVKELSVIDGRRAQNCNILLSRLKLSNDEIKRAILTMDEQEDLPKDMLEQLLKFVPEKSDIDLLEEHKHELDRMAKADRFLFEMSRINHYQQRLQSLYFKKKFAERVAEVKPKVEAIRSGSEEVFRSGALKQLLEVVLAFGNYMNKGQRGNAYGFKISSLNKIADTKSSIDKNITLLHYLITIVENKYPSVLNLNEELRDIPQAAKVNMTELDKEISTLRSGLKAVETELEYQKSQPPQPGDKFVSVVSQFITVASFSFSDVEDLLAEAKDLFTKAVKHFGEEAGKIQPDEFFGIFDQFLQAVSEAKQENENMRKKKEEEERRARMEAQLKEQRERERKMRKAKENSEESGEFDDLVSALRSGEVFDKDLSKLKRNRKRITNQMTDSSRERPITKLNF |
| 预测分子量 | 123,4 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是3篇关于DAAM1重组蛋白的参考文献示例(内容为虚构，供格式参考)：
1. **文献名称**: "Recombinant DAAM1 regulates actin assembly via formin homology domains"
**作者**: Smith J, et al.
**摘要**: 本研究利用大肠杆菌表达系统成功纯化了重组DAAM1蛋白，并通过体外实验证明其通过formin结构域促进肌动蛋白纤维的成核和延伸，揭示了DAAM1在细胞骨架动态调控中的分子机制。
2. **文献名称**: "Structural analysis of DAAM1 in Wnt/PCP signaling using cryo-EM"
**作者**: Chen L, Wang H.
**摘要**: 通过冷冻电镜技术解析了重组DAAM1蛋白与Dishevelled蛋白复合物的三维结构，阐明了DAAM1在Wnt平面细胞极性(PCP)通路中协调信号转导的结构基础。
3. **文献名称**: "DAAM1 recombinant protein facilitates microtubule stabilization in neuronal development"
**作者**: Gonzalez R, et al.
**摘要**: 研究显示，体外重组的DAAM1蛋白能够与微管结合并增强其稳定性，实验表明DAAM1通过调控微管-肌动蛋白交联参与神经元轴突生长的极性建立。
---
注：以上文献为示例性内容，实际引用请以真实发表的论文为准。如需查找真实文献，建议在PubMed或Web of Science中检索关键词“DAAM1 recombinant protein”或“DAAM1 expression”。
**Background of DAAM1 Recombinant Protein**
DAAM1 (Dishevelled-Associated Activator of Morphogenesis 1) is a member of the formin family of proteins, which are critical regulators of actin cytoskeleton dynamics. Initially identified for its interaction with Dishevelled (Dvl) in the Wnt/planar cell polarity (PCP) signaling pathway, DAAM1 plays a pivotal role in cell polarity, migration, and tissue morphogenesis. Structurally, DAAM1 contains formin homology domains (FH1 and FH2) that enable its function in nucleating and elongating unbranched actin filaments, a process essential for cellular processes like cytokinesis, vesicle trafficking, and cell adhesion.
Recombinant DAAM1 protein is engineered through molecular cloning techniques, typically expressed in *E. coli* or mammalian systems, to produce a purified, functional form for experimental studies. This recombinant tool allows researchers to dissect DAAM1’s biochemical properties, such as its actin polymerization activity, and its interplay with signaling partners like Rho GTPases or Dvl. Studies using recombinant DAAM1 have revealed its role in coordinating cytoskeletal remodeling during embryogenesis, neural development, and cancer cell invasiveness. For instance, DAAM1 is implicated in axon guidance and neural tube closure via its regulation of actin dynamics in response to Wnt-PCP signals.
The protein’s involvement in pathological contexts, such as metastatic cancers and neurodegenerative disorders, has spurred interest in DAAM1 as a potential therapeutic target. Recombinant DAAM1 serves as a vital reagent for screening inhibitors or studying mutations linked to developmental defects. Its application spans structural biology, cell signaling assays, and drug discovery, making it a cornerstone in understanding cytoskeleton-mediated cellular mechanisms.
×
