| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | CDY1 |
| Uniprot No | Q9Y6F8 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 2-90aa |
| 氨基酸序列 | MHHHHHHASQEFEVEAIVDKRQDKNGNTQYLVRWKGYDKQDDTWEPEQHL MNCEKCVHDFNRRQTEKQKKLTWTTTSRIFSNNARRRTSRSTKANY |
| 预测分子量 | 12 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于CDY1重组蛋白的3篇参考文献及其摘要概括：
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1. **文献名称**: *"Expression and functional analysis of recombinant CDY1 protein in mammalian cells"*
**作者**: Zhang L, et al.
**摘要**: 本研究通过哺乳动物表达系统成功表达并纯化了CDY1重组蛋白，验证了其在体外对组蛋白H3K9乙酰化的调控功能，揭示了CDY1在染色质修饰中的潜在作用机制。
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2. **文献名称**: *"Structural characterization of CDY1 chromodomain and its interaction with histone H3"*
**作者**: Patel RK, et al.
**摘要**: 通过X射线晶体学解析了重组CDY1蛋白的染色质结构域(chromodomain)的三维结构，并证明其与组蛋白H3甲基化位点的特异性结合，为理解CDY1在精子发生中的表观遗传调控提供了结构基础。
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3. **文献名称**: *"CDY1 recombinant protein enhances spermatogenesis in azoospermic mouse models"*
**作者**: Chen H, et al.
**摘要**: 利用原核系统表达CDY1重组蛋白，并在无精症小鼠模型中验证其通过调控减数分裂相关基因表达恢复精子生成的潜力，表明CDY1可能成为男性不育治疗的靶点。
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**备注**：以上文献信息为模拟概括，实际研究中建议通过PubMed或Web of Science等数据库检索具体文献。
CDY1 (Chromodomain protein Y-linked 1) is a male-specific protein encoded by a gene located on the Y chromosome within the AZFc (azoospermia factor c) region, a critical locus associated with spermatogenesis and male fertility. As a member of the CDY family, CDY1 contains a chromodomain that facilitates chromatin interaction and a catalytic domain with histone acetyltransferase (HAT) activity. It plays a pivotal role in chromatin remodeling during spermiogenesis, particularly in the histone-to-protamine transition, a process essential for sperm head condensation and DNA compaction. CDY1 is implicated in regulating gene expression by acetylating histones, thereby modifying chromatin structure and enabling transcriptional activation or repression.
The CDY1 gene is evolutionarily conserved in primates and exhibits testis-specific expression. Studies suggest its involvement in male germ cell development, with mutations or deletions in the AZFc region (including CDY1) linked to severe oligospermia or azoospermia. Recombinant CDY1 protein, typically produced in bacterial or eukaryotic expression systems using genetic engineering techniques, retains its HAT activity and chromatin-binding properties. This recombinant form is widely utilized in functional studies to elucidate molecular mechanisms in chromatin dynamics, histone modification, and epigenetic regulation during spermatogenesis. It also serves as a tool for developing diagnostic assays for Y chromosome-related infertility and exploring potential therapeutic targets for male reproductive disorders. Recent research has expanded its applications to include studies on epigenetic inheritance and chromatin-related diseases beyond reproductive biology.
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