| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | SLC25A13 |
| Uniprot No | Q9UJS0 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-675aa |
| 氨基酸序列 | MAAAKVALTKRADPAELRTIFLKYASIEKNGEFFMSPNDFVTRYLNIFGESQPNPKTVELLSGVVDQTKDGLISFQEFVAFESVLCAPDALFMVAFQLFDKAGKGEVTFEDVKQVFGQTTIHQHIPFNWDSEFVQLHFGKERKRHLTYAEFTQFLLEIQLEHAKQAFVQRDNARTGRVTAIDFRDIMVTIRPHVLTPFVEECLVAAAGGTTSHQVSFSYFNGFNSLLNNMELIRKIYSTLAGTRKDVEVTKEEFVLAAQKFGQVTPMEVDILFQLADLYEPRGRMTLADIERIAPLEEGTLPFNLAEAQRQKASGDSARPVLLQVAESAYRFGLGSVAGAVGATAVYPIDLVKTRMQNQRSTGSFVGELMYKNSFDCFKKVLRYEGFFGLYRGLLPQLLGVAPEKAIKLTVNDFVRDKFMHKDGSVPLAAEILAGGCAGGSQVIFTNPLEIVKIRLQVAGEITTGPRVSALSVVRDLGFFGIYKGAKACFLRDIPFSAIYFPCYAHVKASFANEDGQVSPGSLLLAGAIAGMPAASLVTPADVIKTRLQVAARAGQTTYSGVIDCFRKILREEGPKALWKGAGARVFRSSPQFGVTLLTYELLQRWFYIDFGGVKPMGSEPVPKSRINLPAPNPDHVGGYKLAVATFAGIENKFGLYLPLFKPSVSTSKAIGGGP |
| 预测分子量 | 74,1 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于SLC25A13重组蛋白的3篇代表性文献示例(注：文献信息为模拟概括，实际引用需核实)：
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1. **文献名称**：*Functional characterization of the SLC25A13 protein as a mitochondrial aspartate/glutamate carrier*
**作者**：Palmieri F, et al.
**摘要**：通过在大肠杆菌中重组表达SLC25A13蛋白，研究其在线粒体膜上的转运活性，证实其作为天冬氨酸/谷氨酸载体的功能，并分析pH与钙离子对其转运效率的影响。
2. **文献名称**：*Expression and purification of recombinant SLC25A13 for structural studies*
**作者**：Kobayashi K, et al.
**摘要**：报道了在哺乳动物细胞系统中高效表达和纯化人源SLC25A13重组蛋白的方法，利用冷冻电镜技术初步解析其三维结构，探讨关键结构域与底物结合的关系。
3. **文献名称**：*Mutational analysis of SLC25A13 in Citrin deficiency using recombinant protein models*
**作者**：Saheki T, et al.
**摘要**：通过构建携带Citrin缺陷症相关突变的SLC25A13重组蛋白，在酵母模型中进行功能互补实验，揭示特定突变导致转运活性丧失的分子机制。
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**建议**：实际文献需通过PubMed或Google Scholar检索关键词“SLC25A13 recombinant protein”“Citrin”“aspartate/glutamate carrier”等验证。重点关注领域内权威期刊(如*Biochimica et Biophysica Acta*、*Human Molecular Genetics*)及团队(如Palmieri、Saheki课题组)。
SLC25A13 is a nuclear-encoded gene located on chromosome 7q21.3 that encodes citrin, a mitochondrial carrier protein primarily expressed in the liver, kidneys, and pancreas. As a member of the solute carrier family 25 (SLC25), citrin facilitates the transport of aspartate, glutamate, and other metabolites across the mitochondrial inner membrane, playing a critical role in the urea cycle, gluconeogenesis, and amino acid metabolism. Structurally, citrin consists of 675 amino acids and features six transmembrane domains characteristic of mitochondrial carrier proteins.
Recombinant SLC25A13 protein is engineered to study its biochemical functions and pathological implications. Citrin deficiency, caused by SLC25A13 mutations, is linked to two autosomal recessive disorders: neonatal intrahepatic cholestasis (NICCD) and adult-onset type II citrullinemia (CTLN2). These conditions highlight citrin's role in maintaining metabolic homeostasis, particularly in ammonia detoxification and energy balance. Recombinant protein production typically involves expression systems like E. coli or mammalian cell lines, followed by purification via affinity tags.
Research on SLC25A13 recombinant protein aids in elucidating transport mechanisms, substrate specificity, and mutation-induced functional disruptions. It also serves as a tool for developing diagnostic assays or therapeutic strategies targeting citrin deficiency. Additionally, studies using recombinant citrin help explore its interaction with other metabolic enzymes, such as argininosuccinate synthase, to unravel broader regulatory networks in mitochondrial metabolism. This protein's unique role in bridging mitochondrial and cytosolic pathways underscores its importance in both basic research and clinical applications.
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