| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | NUSAP1 |
| Uniprot No | Q9BXS6 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-441aa |
| 氨基酸序列 | MIIPSLEELD SLKYSDLQNL AKSLGLRANL RATKLLKALK GYIKHEARKG NENQDESQTS ASSCDETEIQ ISNQEEAERQ PLGHVTKTRR RCKTVRVDPD SQQNHSEIKI SNPTEFQNHE KQESQDLRAT AKVPSPPDEH QEAENAVSSG NRDSKVPSEG KKSLYTDESS KPGKNKRTAI TTPNFKKLHE AHFKEMESID QYIERKKKHF EEHNSMNELK QQPINKGGVR TPVPPRGRLS VASTPISQRR SQGRSCGPAS QSTLGLKGSL KRSAISAAKT GVRFSAATKD NEHKRSLTKT PARKSAHVTV SGGTPKGEAV LGTHKLKTIT GNSAAVITPF KLTTEATQTP VSNKKPVFDL KASLSRPLNY EPHKGKLKPW GQSKENNYLN QHVNRINFYK KTYKQPHLQT KEEQRKKREQ ERKEKKAKVL GMRRGLILAE D |
| 预测分子量 | 49,4 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于NUSAP1重组蛋白的3篇代表性文献(注：以下内容为示例性概括，具体文献需根据实际检索结果调整)：
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1. **文献名称**：*"Recombinant NUSAP1 promotes mitotic progression and interacts with microtubule networks"*
**作者**：Smith A, et al.
**摘要**：研究通过大肠杆菌系统成功表达并纯化重组NUSAP1蛋白，证实其通过稳定微管结构促进有丝分裂进程，并揭示了其与纺锤体微管的直接相互作用机制。
2. **文献名称**：*"NUSAP1 knockdown impairs DNA damage repair: Insights from recombinant protein functional assays"*
**作者**：Chen X, et al.
**摘要**：利用重组NUSAP1蛋白进行体外功能实验，发现其通过结合损伤DNA并招募BRCA1蛋白，增强细胞对电离辐射的修复能力，提示其在基因组稳定性中的关键作用。
3. **文献名称**：*"Structural basis of NUSAP1's role in chromosome segregation: A cryo-EM study"*
**作者**：Tanaka K, et al.
**摘要**：通过冷冻电镜解析重组NUSAP1蛋白的三维结构，揭示其C端结构域与染色体结合的关键区域，为理解其调控染色体分离的分子机制提供结构基础。
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**备注**：以上文献为假设性示例，实际研究中建议通过PubMed、Web of Science等数据库检索真实文献(关键词：NUSAP1 recombinant protein / expression / function)。若需具体文献协助，可提供研究领域方向(如癌症、细胞周期等)，可进一步优化推荐。
**Background of NUSAP1 Recombinant Protein**
Nucleolar and spindle-associated protein 1 (NUSAP1) is a microtubule-binding protein implicated in mitotic regulation, genome stability, and cancer progression. It belongs to the conserved NUSAP family, characterized by a unique N-terminal nucleolar localization signal, a central microtubule-binding domain, and a C-terminal chromatin-binding region. NUSAP1 is predominantly expressed during the G2/M phase of the cell cycle, where it localizes to the mitotic spindle, kinetochores, and chromosomes, ensuring proper chromosome segregation, spindle assembly, and microtubule dynamics. Dysregulation of NUSAP1 disrupts mitotic fidelity, leading to chromosomal instability—a hallmark of cancer.
The recombinant NUSAP1 protein is typically produced using expression systems like *E. coli* or mammalian cells, enabling studies on its biochemical properties and interactions. Purified recombinant NUSAP1 retains functional domains, allowing researchers to investigate its roles in microtubule stabilization, DNA repair, and interactions with partners such as TPX2 and Aurora kinases.
NUSAP1 is overexpressed in multiple cancers, including prostate, breast, and hepatocellular carcinomas, correlating with poor prognosis. It promotes tumorigenesis by enhancing cell proliferation, invasion, and metastasis, potentially through pathways like PI3K/AKT and Wnt/β-catenin. Recombinant NUSAP1 serves as a critical tool for elucidating these mechanisms, screening anticancer drugs, and developing diagnostic or therapeutic strategies targeting its oncogenic functions. Its study bridges mitotic biology and cancer research, offering insights into precision oncology approaches.
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