| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | FZD9 |
| Uniprot No | O00144 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-591aa |
| 氨基酸序列 | MAVAPLRGALLLWQLLAAGGAALEIGRFDPERGRGAAPCQAVEIPMCRGIGYNLTRMPNLLGHTSQGEAAAELAEFAPLVQYGCHSHLRFFLCSLYAPMCTDQVSTPIPACRPMCEQARLRCAPIMEQFNFGWPDSLDCARLPTRNDPHALCMEAPENATAGPAEPHKGLGMLPVAPRPARPPGDLGPGAGGSGTCENPEKFQYVEKSRSCAPRCGPGVEVFWSRRDKDFALVWMAVWSALCFFSTAFTVLTFLLEPHRFQYPERPIIFLSMCYNVYSLAFLIRAVAGAQSVACDQEAGALYVIQEGLENTGCTLVFLLLYYFGMASSLWWVVLTLTWFLAAGKKWGHEAIEAHGSYFHMAAWGLPALKTIVILTLRKVAGDELTGLCYVASTDAAALTGFVLVPLSGYLVLGSSFLLTGFVALFHIRKIMKTGGTNTEKLEKLMVKIGVFSILYTVPATCVIVCYVYERLNMDFWRLRATEQPCAAAAGPGGRRDCSLPGGSVPTVAVFMLKIFMSLVVGITSGVWVWSSKTFQTWQSLCYRKIAAGRARAKACRAPGSYGRGTHCHYKAPTVVLHMTKTDPSLENPTHL |
| 预测分子量 | 64,4 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于FZD9重组蛋白的3篇代表性文献(信息基于公开研究整理，非真实文献，仅供示例参考)：
1. **文献名称**：*Structural insights into FZD9 receptor activation by recombinant Wnt proteins*
**作者**：Smith A, et al.
**摘要**：研究通过重组表达纯化FZD9蛋白及其配体Wnt3a，利用冷冻电镜解析了FZD9与Wnt3a的复合物结构，揭示了其跨膜信号传导的分子机制。
2. **文献名称**：*FZD9 recombinant protein promotes neural stem cell proliferation via Wnt/β-catenin pathway*
**作者**：Zhang L, et al.
**摘要**：体外实验表明，重组FZD9蛋白通过激活Wnt/β-catenin通路增强神经干细胞的自我更新能力，提示其在神经再生治疗中的潜在应用价值。
3. **文献名称**：*Targeting FZD9 with a recombinant antagonist inhibits leukemia progression*
**作者**：Chen H, et al.
**摘要**：开发了一种重组FZD9拮抗剂蛋白，在急性髓系白血病模型中有效阻断Wnt信号异常激活，显著抑制肿瘤细胞生长并延长小鼠生存期。
如需真实文献，建议通过PubMed或Google Scholar检索关键词“FZD9 recombinant protein”或“FZD9 expression and function”。
FZD9 (Frizzled class receptor 9) is a member of the Frizzled family of G protein-coupled receptors, which play critical roles in the Wnt signaling pathway. This evolutionarily conserved pathway regulates embryonic development, tissue homeostasis, and stem cell maintenance. As a transmembrane receptor, FZD9 binds Wnt ligands through its extracellular cysteine-rich domain (CRD), initiating both canonical (β-catenin-dependent) and non-canonical (β-catenin-independent) signaling cascades. Its structure features seven transmembrane helices and a conserved intracellular motif for downstream signal transduction.
Recombinant FZD9 protein is engineered for research and therapeutic applications, typically expressed in mammalian or bacterial systems with purification tags like His or Fc. This engineered protein retains functional domains to study receptor-ligand interactions, screen drug candidates, or modulate Wnt signaling in disease models. Notably, FZD9 is implicated in cancer progression, neurodevelopmental disorders, and bone metabolism. Aberrant expression has been observed in leukemia, colorectal cancer, and Alzheimer's disease, making it a potential therapeutic target.
The production of recombinant FZD9 enables structural studies (e.g., X-ray crystallography of CRD-Wnt complexes) and functional assays to dissect signaling mechanisms. Researchers use it to develop antibody therapies or small molecules that either enhance or inhibit Wnt pathway activity. Current challenges include maintaining proper post-translational modifications and conformational stability during recombinant production. Ongoing studies focus on optimizing expression systems to preserve native receptor functionality while ensuring high yield for biomedical applications.
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