| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | CEP57 |
| Uniprot No | Q86XR8 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-500aa |
| 氨基酸序列 | MAAASVSAAS GSHLSNSFAE PSRSNGSMVR HSSSPYVVYP SDKPFLNSDL RRSPSKPTLA YPESNSRAIF SALKNLQDKI RRLELERIQA EESVKTLSRE TIEYKKVLDE QIQERENSKN EESKHNQELT SQLLAAENKC NLLEKQLEYM RNMIKHAEME RTSVLEKQVS LERERQHDQT HVQSQLEKLD LLEQEYNKLT TMQALAEKKM QELEAKLHEE EQERKRMQAK AAELQTGLET NRLIFEDKAT PCVPNARRIK KKKSKPPEKK SSRNYFGAQP HYRLCLGDMP FVAGKSTSPS HAVVANVQLV LHLMKQHSKA LCNDRVINSI PLAKQVSSRG GKSKKLSVTP PSSNGINEEL SEVLQTLQDE FGQMSFDHQQ LAKLIQESPT VELKDKLECE LEALVGRMEA KANQITKVRK YQAQLEKQKL EKQKKELKAT KKTLDEERNS SSRSGITGTT NKKDFMKLRP GEKRRKNLQL LKDMQSIQNS LQSSSLCWDY |
| 预测分子量 | 57 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
1. **"CEP57 is required for centrosome duplication in human cells"**
- Authors: Srsen, V., et al.
- 摘要：研究通过重组CEP57蛋白的功能分析，揭示其在中心体复制中的关键作用，并发现CEP57缺失会导致染色体不稳定性，与MOPDII(一种侏儒症)的发病机制相关。
2. **"Structural insights into CEP57’s role in microtubule stabilization"**
- Authors: Sillibourne, J.E., et al.
- 摘要：利用重组CEP57蛋白进行X射线晶体学分析，阐明了其C端结构域与微管结合的分子机制，解释了CEP57突变导致微管组织异常的机理。
3. **"CEP57 mutations disrupt nuclear localization and microtubule cohesion in mosaic variegated aneuploidy syndrome"**
- Authors: Pihan, G.A., et al.
- 摘要：通过重组CEP57突变体实验，证明CEP57的核定位信号对其在细胞分裂中维持基因组稳定性至关重要，突变会导致染色体错误分离。
4. **"Recombinant CEP57 characterization reveals phosphorylation-dependent oligomerization"**
- Authors: Fry, A.M., et al.
- 摘要：研究重组CEP57的生化特性，发现其通过磷酸化调控形成多聚体结构，进而影响中心体微管锚定功能，为靶向治疗提供潜在策略。
(注：以上文献信息为示例性概括，实际引用需核实具体文献。)
**Background of CEP57 Recombinant Protein**
CEP57 (Centrosomal Protein 57), also known as TSPYL1. is a multifunctional protein encoded by the *CEP57* gene. It plays a critical role in maintaining genomic stability, primarily through its involvement in centrosome duplication, microtubule organization, and mitotic spindle assembly during cell division. CEP57 acts as a scaffold, facilitating interactions between key centrosomal components and ensuring proper segregation of chromosomes. Dysregulation of CEP57 is linked to chromosomal instability, a hallmark of cancer, and mutations in this gene are associated with mosaic variegated aneuploidy (MVA) syndrome, a rare autosomal recessive disorder characterized by developmental delays and cancer predisposition.
The recombinant CEP57 protein is engineered using molecular cloning techniques, typically expressed in *E. coli* or mammalian cell systems to ensure proper folding and post-translational modifications. It retains the functional domains of native CEP57. including its coiled-coil regions and microtubule-binding sites, enabling its use in *in vitro* studies. Researchers employ CEP57 recombinant protein to investigate its interactions with partner proteins (e.g., CEP63. CEP152), its role in DNA repair mechanisms, and its ability to stabilize the mitotic apparatus.
Additionally, recombinant CEP57 serves as a tool for screening therapeutic agents targeting centrosomal abnormalities in cancers or for functional studies in disease models. Its availability accelerates research into the molecular basis of aneuploidy-related disorders and potential interventions. By elucidating CEP57's structural and mechanistic roles, this recombinant protein contributes to broader insights into cell cycle regulation and oncogenesis.
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