| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | IPO5 |
| Uniprot No | O00410 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-1097aa |
| 氨基酸序列 | MAAAAAEQQQFYLLLGNLLSPDNVVRKQAEETYENIPGQSKITFLLQAIRNTTAAEEARQMAAVLLRRLLSSAFDEVYPALPSDVQTAIKSELLMIIQMETQSSMRKKVCDIAAELARNLIDEDGNNQWPEGLKFLFDSVSSQNVGLREAALHIFWNFPGIFGNQQQHYLDVIKRMLVQCMQDQEHPSIRTLSARATAAFILANEHNVALFKHFADLLPGFLQAVNDSCYQNDDSVLKSLVEIADTVPKYLRPHLEATLQLSLKLCGDTSLNNMQRQLALEVIVTLSETAAAMLRKHTNIVAQTIPQMLAMMVDLEEDEDWANADELEDDDFDSNAVAGESALDRMACGLGGKLVLPMIKEHIMQMLQNPDWKYRHAGLMALSAIGEGCHQQMEGILNEIVNFVLLFLQDPHPRVRYAACNAVGQMATDFAPGFQKKFHEKVIAALLQTMEDQGNQRVQAHAAAALINFTEDCPKSLLIPYLDNLVKHLHSIMVLKLQELIQKGTKLVLEQVVTSIASVADTAEEKFVPYYDLFMPSLKHIVENAVQKELRLLRGKTIECISLIGLAVGKEKFMQDASDVMQLLLKTQTDFNDMEDDDPQISYMISAWARMCKILGKEFQQYLPVVMGPLMKTASIKPEVALLDTQDMENMSDDDGWEFVNLGDQQSFGIKTAGLEEKSTACQMLVCYAKELKEGFVEYTEQVVKLMVPLLKFYFHDGVRVAAAESMPLLLECARVRGPEYLTQMWHFMCDALIKAIGTEPDSDVLSEIMHSFAKCIEVMGDGCLNNEHFEELGGILKAKLEEHFKNQELRQVKRQDEDYDEQVEESLQDEDDNDVYILTKVSDILHSIFSSYKEKVLPWFEQLLPLIVNLICPHRPWPDRQWGLCIFDDVIEHCSPASFKYAEYFLRPMLQYVCDNSPEVRQAAAYGLGVMAQYGGDNYRPFCTEALPLLVRVIQSADSKTKENVNATENCISAVGKIMKFKPDCVNVEEVLPHWLSWLPLHEDKEEAVQTFNYLCDLIESNHPIVLGPNNTNLPKIFSIIAEGEMHEAIKHEDPCAKRLANVVRQVQTSGGLWTECIAQLSPEQQAAIQELLNSA |
| 预测分子量 | 123,6 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是3篇关于IPO5重组蛋白的相关文献示例(部分信息基于研究领域典型内容模拟)：
1. **文献名称**：Structural insights into the nuclear transport mechanism of IPO5 by recombinant protein analysis
**作者**：Zhang Y, et al.
**摘要**：本研究利用重组表达的IPO5蛋白解析其核转运结构域的三维构象，揭示了其与核孔复合体相互作用的关键氨基酸位点，为理解核质运输机制提供结构基础。
2. **文献名称**：Functional characterization of recombinant IPO5 in HIV-1 viral protein nuclear import
**作者**：Lee SM, et al.
**摘要**：通过体外重组IPO5蛋白实验，证实其在HIV-1整合酶蛋白核转运中的关键作用，并发现其与病毒复制效率的正相关性，为抗病毒靶点开发提供依据。
3. **文献名称**：Optimization of recombinant IPO5 expression in E. coli for biochemical assays
**作者**：Gupta R, et al.
**摘要**：系统优化了IPO5重组蛋白在大肠杆菌中的可溶性表达条件，建立了高效纯化流程，并验证其在体外核转运活性分析中的应用可行性。
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**备注**：上述文献为领域典型研究方向模拟，实际文献需通过PubMed或Web of Science检索确认。建议使用关键词 "IPO5 recombinant protein nuclear transport" 或 "Importin-5 expression and purification" 进行精准查询。
**Background of IPO5 Recombinant Protein**
IPO5 (Importin-5), also known as RanBP5. is a member of the importin β superfamily of nuclear transport receptors. It plays a critical role in the nucleocytoplasmic transport of macromolecules, facilitating the movement of specific cargo proteins and RNA molecules between the cytoplasm and nucleus. IPO5 functions by recognizing nuclear localization signals (NLS) or other cargo-specific motifs, interacting with the Ran GTPase cycle to ensure directional transport. Structurally, it contains characteristic HEAT repeats that mediate cargo binding and regulatory interactions.
The recombinant IPO5 protein is engineered using heterologous expression systems (e.g., *E. coli*, insect cells, or mammalian cells*) to produce a purified, functional form for research applications. Its recombinant version retains the ability to bind cargoes such as ribosomal proteins, transcription factors, and viral components, making it valuable for studying nuclear import mechanisms, viral-host interactions, and cellular signaling pathways.
IPO5 has been implicated in diseases, including cancer and viral infections, where dysregulated nuclear transport contributes to pathogenesis. For example, it interacts with HIV-1 integrase and influenza virus nucleoproteins, aiding viral replication. Recombinant IPO5 is also used to develop assays for screening nuclear transport inhibitors or studying gene regulation.
In biotechnology, recombinant IPO5 is optimized for high purity and activity, often tagged with markers (e.g., His, GST) for detection and purification. Quality control involves SDS-PAGE, Western blotting, and functional assays (e.g., cargo-binding validation). Its study advances understanding of cellular trafficking and supports therapeutic development targeting transport-related disorders.
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