| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | CHSY1 |
| Uniprot No | Q86X52 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-802aa |
| 氨基酸序列 | MAARGRRAWLSVLLGLVLGFVLASRLVLPRASELKRAGPRRRASPEGCRSGQAAASQAGGARGDARGAQLWPPGSDPDGGPRDRNFLFVGVMTAQKYLQTRAVAAYRTWSKTIPGKVQFFSSEGSDTSVPIPVVPLRGVDDSYPPQKKSFMMLKYMHDHYLDKYEWFMRADDDVYIKGDRLENFLRSLNSSEPLFLGQTGLGTTEEMGKLALEPGENFCMGGPGVIMSREVLRRMVPHIGKCLREMYTTHEDVEVGRCVRRFAGVQCVWSYEMQQLFYENYEQNKKGYIRDLHNSKIHQAITLHPNKNPPYQYRLHSYMLSRKISELRHRTIQLHREIVLMSKYSNTEIHKEDLQLGIPPSFMRFQPRQREEILEWEFLTGKYLYSAVDGQPPRRGMDSAQREALDDIVMQVMEMINANAKTRGRIIDFKEIQYGYRRVNPMYGAEYILDLLLLYKKHKGKKMTVPVRRHAYLQQTFSKIQFVEHEELDAQELAKRINQESGSLSFLSNSLKKLVPFQLPGSKSEHKEPKDKKINILIPLSGRFDMFVRFMGNFEKTCLIPNQNVKLVVLLFNSDSNPDKAKQVELMRDYRIKYPKADMQILPVSGEFSRALALEVGSSQFNNESLLFFCDVDLVFTTEFLQRCRANTVLGQQIYFPIIFSQYDPKIVYSGKVPSDNHFAFTQKTGFWRNYGFGITCIYKGDLVRVGGFDVSIQGWGLEDVDLFNKVVQAGLKTFRSQEVGVVHVHHPVFCDPNLDPKQYKMCLGSKASTYGSTQQLAEMWLEKNDPSYSKSSNNNGSVRTA |
| 预测分子量 | 91,7 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于CHSY1重组蛋白的3篇文献摘要简述：
1. **文献名称**：*"Functional analysis of chondroitin sulfate synthase 1 in zebrafish development"*
**作者**：Izumikawa T, et al.
**摘要**：通过重组CHSY1蛋白体外实验，揭示了其催化硫酸软骨素链合成的关键酶活性，并利用斑马鱼模型证明CHSY1缺失导致骨骼发育异常。
2. **文献名称**：*"Recombinant human CHSY1 expression and its role in cancer cell migration"*
**作者**：Wang H, et al.
**摘要**：研究重组人源CHSY1蛋白在肿瘤细胞中的过表达效应，发现其通过调节细胞外基质中硫酸软骨素含量促进癌细胞迁移和侵袭。
3. **文献名称**：*"Structural characterization of recombinant CHSY1 and its interaction with glycosaminoglycan substrates"*
**作者**：Yada T, et al.
**摘要**：利用重组CHSY1蛋白进行结构解析，明确了其底物结合域和催化机制，为设计靶向硫酸软骨素合成的抑制剂提供理论依据。
以上文献聚焦于CHSY1重组蛋白的酶活性、病理功能及结构分析，涵盖发育生物学和疾病机制研究。
CHSY1 (Chondroitin Sulfate Synthase 1) is a key enzyme in the biosynthesis of chondroitin sulfate, a sulfated glycosaminoglycan (GAG) widely distributed in the extracellular matrix and cell surfaces. As a member of the glycosyltransferase family, CHSY1 catalyzes the transfer of glucuronic acid and N-acetylgalactosamine residues to form the repeating disaccharide backbone of chondroitin, a precursor of chondroitin sulfate. This enzymatic activity is critical for maintaining the structural integrity of connective tissues, regulating cell signaling, and modulating interactions between cells and their microenvironment.
The CHSY1 gene is located on chromosome 15q26.3 and encodes a transmembrane protein predominantly expressed in cartilage, brain, and other tissues rich in extracellular matrix. Mutations in CHSY1 are associated with autosomal recessive Temtamy syndrome, characterized by skeletal dysplasia, intellectual disability, and ocular abnormalities, underscoring its biological significance in development and homeostasis.
Recombinant CHSY1 protein is produced using heterologous expression systems (e.g., E. coli, mammalian cells) to enable functional studies and therapeutic applications. Its purified form allows researchers to investigate enzyme kinetics, substrate specificity, and interactions with other biosynthetic enzymes or pharmaceutical compounds. In drug development, recombinant CHSY1 serves as a tool for screening inhibitors or modulators targeting chondroitin sulfate-related pathologies, including osteoarthritis, cancer metastasis, and neurodegenerative disorders. Additionally, it aids in structural studies using X-ray crystallography or cryo-EM to elucidate catalytic mechanisms, supporting rational design of therapeutics. Recent studies also explore its role in synthetic biology for engineering customized chondroitin sulfate chains with tailored biological properties.
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