| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | UBA6 |
| Uniprot No | A0AVT1-3 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-389aa |
| 氨基酸序列 | MEGSEPVAAHQGEEASCSSWGTGSTNKNLPIMSTASVEIDDALYSRQRYV LGDTAMQKMAKSHVFLSGMGGLGLEIAKNLVLAGIKAVTIHDTEKCQAWD LGTNFFLSEDDVVNKRNRAEAVLKHIAELNPYVHVTSSSVPFNETTDLSF LDKYQCVVLTEMKLPLQKKINDFCRSQCPPIKFISADVHGIWSRLFCDFG DEFEVLDTTGEEPKEIFISNITQANPGIVTCLENHPHKLETGQFLTFREI NGMTGLNGSIQQITVISPFSFSIGDTTELEPYLHGGIAVQVKTPKTVFFE SLERQLKHPKCLIVDFSNPEAPLEIHTAMLALDQFQEKYSRKPNVGCQQD SEELLKLATSISETLEEKVTIEIYGCPNICLLIHKCSVY |
| 预测分子量 | 68 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于UBA6重组蛋白的3篇代表性文献示例(内容基于领域研究主题，仅供参考)：
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1. **文献名称**: *"UBA6 Regulates Protein Ubiquitination and Cell Survival via Distinct Mechanisms"*
**作者**: Chen et al.
**摘要**: 本研究通过在大肠杆菌系统中重组表达并纯化UBA6蛋白，结合基因敲除细胞模型，揭示了UBA6通过激活特定泛素结合酶(E2)调控蛋白质稳定性，并参与细胞应激反应通路的关键作用。
2. **文献名称**: *"Structural Insights into the Activation of UBA6 by the Ubiquitin-like Protein FAT10"*
**作者**: Pelzer et al.
**摘要**: 利用X射线晶体学解析了重组UBA6与FAT10蛋白的复合物结构，阐明了两者结合的分子机制，证明FAT10可通过变构效应增强UBA6的酶活性，为泛素化修饰的交叉调控提供结构依据。
3. **文献名称**: *"Development of a High-Throughput Assay for UBA6-Dependent Ubiquitination"*
**作者**: Huang et al.
**摘要**: 报道了一种基于重组UBA6蛋白的体外泛素化检测方法，通过荧光标记技术实现快速定量分析，验证了UBA6在多种底物泛素化中的特异性，并筛选出小分子抑制剂以探究其治疗潜力。
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**注**：以上文献为示例，具体研究需以实际论文为准。建议通过PubMed或Web of Science检索关键词“UBA6 recombinant protein”或“UBA6 ubiquitination”获取最新文献。
UBA6 (Ubiquitin-like modifier-activating enzyme 6) is a member of the E1 ubiquitin-activating enzyme family, which plays a critical role in initiating the ubiquitination cascade—a post-translational modification process essential for protein degradation, signaling, and cellular regulation. Unlike its well-studied paralog UBA1. UBA6 specifically activates ubiquitin and the ubiquitin-like protein FAT10 (HLA-F adjacent transcript 10), highlighting its unique substrate specificity. This enzyme facilitates the ATP-dependent transfer of ubiquitin/FAT10 to E2 conjugating enzymes, ultimately guiding substrates to proteasomal degradation or non-degradative regulatory pathways.
Recombinant UBA6 protein is typically produced using expression systems like *E. coli* or mammalian cells, enabling researchers to study its structure, enzymatic activity, and interactions in vitro. Its recombinant form retains the ability to form a thioester bond with ubiquitin, a hallmark of functional E1 enzymes. Studies using recombinant UBA6 have revealed its involvement in diverse physiological processes, including immune response, neurodevelopment, and cell cycle control. Dysregulation of UBA6 has been linked to cancers, neurodegenerative disorders (e.g., Alzheimer’s disease), and autoimmune conditions, positioning it as a potential therapeutic target.
The development of recombinant UBA6 has advanced mechanistic insights into the UBA6-FAT10 axis, a pathway implicated in inflammation and MHC-I antigen presentation. Additionally, it aids in screening small-molecule inhibitors for diseases associated with aberrant ubiquitination. Despite progress, questions remain about its tissue-specific roles and crosstalk with other ubiquitination pathways, driving ongoing research using recombinant tools to unravel its full biological significance.
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