| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | MAP1S |
| Uniprot No | Q66K74 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 794-1053aa |
| 氨基酸序列 | LSDSDPVPLAPGAADSDEDTEGFGVPRHDPLPDPLKVPPPLPDPSSICMVDPEMLPPKTARQTENVSRTRKPLARPNSRAAAPKATPVAAAKTKGLAGGDRASRPLSARSEPSEKGGRAPLSRKSSTPKTATRGPSGSASSRPGVSATPPKSPVYLDLAYLPSGSSAHLVDEEFFQRVRALCYVISGQDQRKEEGMRAVLDALLASKQHWDRDLQVTLIPTFDSVAMHTWYAETHARHQALGITVLGSNSMVSMQDDAFP |
| 预测分子量 | 31.7 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是3条关于MAP1S重组蛋白的模拟参考文献示例(仅供学术参考，非真实文献)：
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1. **文献名称**: *MAP1S regulates autophagosome trafficking by binding to LC3 and end-binding proteins*
**作者**: Xu, Y. et al.
**摘要**: 研究揭示了MAP1S重组蛋白通过N端结构域与LC3蛋白相互作用，调控自噬体沿微管的定向运输，并证明其缺失导致细胞自噬功能异常。
2. **文献名称**: *Recombinant MAP1S expression enhances mitochondrial clearance in neurodegenerative models*
**作者**: Chen, L. et al.
**摘要**: 利用重组MAP1S蛋白在小鼠神经元中的过表达实验，证明其通过促进受损线粒体自噬(mitophagy)减缓帕金森病模型的神经退行性病变。
3. **文献名称**: *Structural characterization of MAP1S and its interaction with tubulin*
**作者**: Wang, H. et al.
**摘要**: 通过X射线晶体学解析了MAP1S重组蛋白的C端结构域，发现其直接结合微管蛋白并稳定微管网络，为开发微管靶向药物提供结构基础。
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**注**：以上内容为模拟示例，实际文献需通过PubMed、Google Scholar等平台检索关键词(如"MAP1S recombinant protein"或"MAP1S autophagy")。
**Background of MAP1S Recombinant Protein**
MAP1S (Microtubule-Associated Protein 1S), also known as MAP1B-like or C19ORF5. is a member of the microtubule-associated protein family involved in regulating cytoskeletal dynamics and intracellular transport. It plays a critical role in stabilizing microtubules, which are essential for cell division, organelle trafficking, and maintaining cell shape. Structurally, MAP1S consists of a heavy chain and a light chain (LC1), with the LC1 subunit mediating interactions with autophagy-related proteins, linking microtubule stability to cellular quality control mechanisms like autophagy.
The recombinant MAP1S protein is engineered using expression systems (e.g., *E. coli* or mammalian cells) to produce purified, functional forms of the protein for research. Recombinant technology allows precise control over post-translational modifications, enhancing its utility in studying molecular interactions. Studies highlight MAP1S as a key mediator of the mTOR signaling pathway, where it coordinates autophagy flux by bridging autophagosomes and microtubules for lysosomal degradation. Dysregulation of MAP1S is implicated in neurodegenerative diseases (e.g., Alzheimer’s) and cancer, where disrupted autophagy promotes tumor progression or neuronal cell death.
In research, MAP1S recombinant protein is widely used to investigate its role in cellular stress responses, neuronal development, and disease mechanisms. It also serves as a tool for drug screening, antibody production, and structural studies. Its dual function in cytoskeletal organization and autophagy underscores its potential as a therapeutic target or biomarker for diseases linked to autophagy dysfunction.
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