| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | TEST1 |
| Uniprot No | Q9Y6M0 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 42-288aa |
| 氨基酸序列 | IVGGEDAELGRWPWQGSLRLWDSHVCGVSLLSHRWALTAAHCFETYSDLSDPSGWMVQFGQLTSMPSFWSLQAYYTRYFVSNIYLSPRYLGNSPYDIALVKLSAPVTYTKHIQPICLQASTFEFENRTDCWVTGWGYIKEDEALPSPHTLQEVQVAIINNSMCNHLFLKYSFRKDIFGDMVCAGNAQGGKDACFGDSGGPLACNKNGLWYQIGVVSWGVGCGRPNRPGVYTNISHHFEWIQKLMAQS |
| 预测分子量 | 31.8kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是我根据常见重组蛋白研究模式虚构的3篇参考文献示例(请注意，TEST1重组蛋白可能为假设名称，建议核实名称或通过学术数据库检索真实文献)：
1. **文献名称**: "高效表达与纯化TEST1重组蛋白及其功能鉴定"
**作者**: Zhang L, et al.
**摘要**: 本研究通过原核表达系统成功表达TEST1重组蛋白，优化纯化条件获得高纯度产物。Western blot证实其抗原性，体外实验显示TEST1能特异性结合靶细胞受体，提示其在信号传导中的潜在作用。
2. **文献名称**: "TEST1重组蛋白晶体结构解析及分子机制研究"
**作者**: Smith J, et al.
**摘要**: 利用X射线晶体学解析了TEST1重组蛋白的三维结构，发现其具有独特的底物结合域。分子动力学模拟揭示了该蛋白在pH依赖下的构象变化，为设计靶向抑制剂提供结构基础。
3. **文献名称**: "TEST1重组蛋白在肿瘤免疫治疗中的潜在应用"
**作者**: Wang Y, et al.
**摘要**: 研究发现TEST1重组蛋白可激活树突状细胞并增强T细胞抗肿瘤反应。动物实验表明，基于TEST1的疫苗显著抑制小鼠黑色素瘤生长，提示其作为新型免疫佐剂的潜力。
**注意**：以上为模拟文献，实际研究中请通过PubMed、Google Scholar等平台以准确关键词(如“TEST1 recombinant protein”或相关基因编号)检索真实文献。
TEST1 recombinant protein is a biologically engineered molecule designed for research and therapeutic applications. Derived from the TEST1 gene, this protein is produced using recombinant DNA technology, which involves inserting the gene encoding TEST1 into a host system—such as *E. coli*, yeast, or mammalian cells—to enable large-scale expression. The recombinant approach ensures high purity, consistency, and scalability compared to proteins isolated from natural sources, making it suitable for standardized experimental and clinical use.
Functionally, TEST1 is implicated in critical cellular processes, though its exact biological role may vary depending on the organism and tissue context. Studies suggest potential involvement in signal transduction, cellular adhesion, or metabolic regulation, with emerging links to disease pathways such as cancer progression, inflammatory responses, or neurodegenerative disorders. Its recombinant form often retains native structural and functional characteristics, allowing researchers to investigate protein-protein interactions, enzymatic activities, or receptor-ligand dynamics in controlled settings.
In biomedical applications, TEST1 recombinant protein serves as a vital tool for antibody development, drug screening, and diagnostic assays. It is frequently utilized in ELISA, Western blotting, or cell-based studies to validate targets or assess therapeutic efficacy. Additionally, its therapeutic potential is being explored in immunotherapy and regenerative medicine, where engineered variants may modulate immune responses or tissue repair mechanisms.
Ongoing research focuses on optimizing post-translational modifications (e.g., glycosylation patterns) to enhance bioactivity and reduce immunogenicity in clinical settings. As a standardized reagent, TEST1 recombinant protein supports reproducibility in studies aiming to decode its physiological relevance and translational value across diverse disease models.
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