| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | AF1521 |
| Uniprot No | O28751 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-192aa |
| 氨基酸序列 | MEVLFEAKVGDITLKLAQGDITQYPAKAIVNAANKRLEHGGGVAYAIAKACAGDAGLYTEISKKAMREQFGRDYIDHGEVVVTPAMNLEERGIKYVFHTVGPICSGMWSEELKEKLYKAFLGPLEKAEEMGVESIAFPAVSAGIYGCDLEKVVETFLEAVKNFKGSAVKEVALVIYDRKSAEVALKVFERSL |
| 预测分子量 | 20,9 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于AF1521重组蛋白的3篇模拟参考文献(注：AF1521为假设性名称，实际文献需通过学术数据库查询)：
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1. **文献名称**: *Structural characterization of recombinant AF1521 protein from Escherichia coli*
**作者**: Zhang L, et al.
**摘要**: 本研究利用大肠杆菌表达系统成功表达并纯化了带有His标签的AF1521重组蛋白。通过X射线晶体学解析了其三维结构，揭示了该蛋白潜在的活性位点，为功能研究提供了结构基础。
2. **文献名称**: *AF1521 recombinant protein as a novel antigen for serodiagnosis of bacterial infections*
**作者**: Patel R, Smith J.
**摘要**: 评估AF1521重组蛋白在血清学诊断中的应用，发现其能够特异性识别患者血清中的抗体，表明其在开发快速检测试剂中具有潜在价值。
3. **文献名称**: *Functional analysis of AF1521 in biofilm formation and bacterial virulence*
**作者**: Kim H, et al.
**摘要**: 通过基因敲除和重组蛋白回补实验，证实AF1521在细菌生物膜形成和致病性中起关键作用，为抗感染治疗提供了新靶点。
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如需获取真实文献，建议在PubMed或Google Scholar中搜索“AF1521 recombinant protein”或相关关键词。
AF1521 recombinant protein is derived from the *Archaeoglobus fulgidus* genome, a hyperthermophilic archaeon found in high-temperature environments such as hydrothermal vents. This protein is part of the CRISPR (Clustered Regularly Interspaced Short Palindromic Repeats) system, a prokaryotic adaptive immune mechanism that defends against invading genetic elements like viruses or plasmids. Specifically, AF1521 is classified as a CRISPR-associated (Cas) protein, though its exact functional role within the CRISPR-Cas machinery remains less characterized compared to well-studied counterparts like Cas9 or Cas12.
Genomic studies suggest AF1521 may belong to the Cmr (Cas module RAMP) complex, which typically mediates RNA-guided RNA cleavage in Type III CRISPR systems. The recombinant form of AF1521 is produced via heterologous expression in *E. coli*, enabling biochemical and structural studies. Its thermostable nature, attributed to the extremophilic origin of *A. fulgidus*, makes it a candidate for applications requiring enzyme stability under harsh conditions.
Research on AF1521 has focused on elucidating its interaction with CRISPR RNAs (crRNAs) and target nucleic acids. Structural analyses reveal conserved domains associated with RNA binding and nuclease activity, though catalytic residues and precise mechanisms remain under investigation. Its recombinant version is also explored for biotechnological tools, such as nucleic acid detection or editing, leveraging CRISPR-derived specificity.
Overall, AF1521 represents a model for studying CRISPR diversity and evolution, offering insights into ancient microbial defense systems and potential engineering into molecular tools. Its study bridges extremophile biology, enzymology, and CRISPR technology development.
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