| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | MARS2 |
| Uniprot No | Q96GW9 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 30-593aa |
| 氨基酸序列 | S SGSLSAGDDA CDVRAYFTTP IFYVNAAPHI GHLYSALLAD ALCRHRRLRG PSTAATRFST GTDEHGLKIQ QAAATAGLAP TELCDRVSEQ FQQLFQEAGI SCTDFIRTTE ARHRVAVQHF WGVLKSRGLL YKGVYEGWYC ASDECFLPEA KVTQQPGPSG DSFPVSLESG HPVSWTKEEN YIFRLSQFRK PLQRWLRGNP QAITPEPFHH VVLQWLDEEL PDLSVSRRSS HLHWGIPVPG DDSQTIYVWL DALVNYLTVI GYPNAEFKSW WPATSHIIGK DILKFHAIYW PAFLLGAGMS PPQRICVHSH WTVCGQKMSK SLGNVVDPRT CLNRYTVDGF RYFLLRQGVP NWDCDYYDEK VVKLLNSELA DALGGLLNRC TAKRINPSET YPAFCTTCFP SEPGLVGPSV RAQAEDYALV SAVATLPKQV ADHYDNFRIY KALEAVSSCV RQTNGFVQRH APWKLNWESP VDAPWLGTVL HVALECLRVF GTLLQPVTPS LADKLLSRLG VSASERSLGE LYFLPRFYGH PCPFEGRRLG PETGLLFPRL DQSRTWLVKA HRT |
| 预测分子量 | 66,5 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是3篇模拟的与MARS2重组蛋白相关的参考文献(实际文献需通过学术数据库检索)：
1. **《MARS2重组蛋白在线粒体功能调控中的分子机制研究》**
- 作者：Lee, S. et al.
- 摘要：研究通过构建MARS2重组蛋白表达系统，揭示其通过调控线粒体RNA代谢影响氧化磷酸化功能，并发现其缺失导致线粒体膜电位下降及细胞凋亡增加。
2. **《利用大肠杆菌系统高效表达人源MARS2重组蛋白的优化策略》**
- 作者：Zhang, Y. & Wang, H.
- 摘要：报道了一种通过密码子优化和诱导条件调控在大肠杆菌中高效表达可溶性MARS2重组蛋白的方法，为后续结构功能研究提供高纯度蛋白样本。
3. **《MARS2基因突变与神经退行性疾病的关联及其重组蛋白功能验证》**
- 作者：Garcia, R. et al.
- 摘要：发现MARS2突变体导致其重组蛋白催化活性丧失，通过体外实验证实该突变与线粒体tRNA甲硫酰化缺陷及神经细胞能量代谢障碍直接相关。
注：以上文献为示例性质，具体研究需参考PubMed、Web of Science等平台的真实论文。
**Background of MARS2 Recombinant Protein**
MARS2 (Mitochondrial Aminoacyl-tRNA Synthetase 2) is a nuclear-encoded enzyme critical for mitochondrial protein synthesis. It catalyzes the attachment of methionine to its cognate tRNA (tRNA^(Met)), a vital step in mitochondrial translation. MARS2 is localized to the mitochondrial matrix, where it supports the production of mitochondrial DNA (mtDNA)-encoded proteins, essential components of the electron transport chain (ETC) involved in ATP synthesis. Dysregulation of MARS2 has been linked to mitochondrial disorders, neurodegenerative diseases, and cancer, underscoring its role in cellular energy metabolism and homeostasis.
Recombinant MARS2 protein is engineered using heterologous expression systems (e.g., *E. coli* or mammalian cells) to produce purified, functional enzyme for research. The recombinant form retains the enzymatic activity of native MARS2. enabling studies on mitochondrial translation mechanisms, disease modeling, and drug screening. Structural analyses reveal conserved domains, including the catalytic core and tRNA-binding regions, which are critical for its interaction with tRNA^(Met) and coordination with other mitochondrial translation factors.
Interest in MARS2 has grown due to its association with rare genetic disorders like autosomal recessive spastic ataxia with leukoencephalopathy (ARSAL), caused by mutations in the *MARS2* gene. Recombinant MARS2 protein aids in elucidating pathogenic mechanisms, such as impaired mitochondrial translation leading to oxidative phosphorylation defects and neuronal degeneration. Additionally, it serves as a tool for exploring therapeutic strategies, including gene therapy or small-molecule interventions targeting mitochondrial dysfunction.
In summary, MARS2 recombinant protein is a key reagent for advancing our understanding of mitochondrial biology and its implications in human diseases, bridging basic research with translational applications.
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