| 纯度 | >85%SDS-PAGE. |
| 种属 | Human |
| 靶点 | DNMT3L |
| Uniprot No | Q9UJW3 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-386aa |
| 氨基酸序列 | MAAIPALDPEAEPSMDVILVGSSELSSSVSPGTGRDLIAYEVKANQRNIE DICICCGSLQVHTQHPLFEGGICAPCKDKFLDALFLYDDDGYQSYCSICC SGETLLICGNPDCTRCYCFECVDSLVGPGTSGKVHAMSNWVCYLCLPSSR SGLLQRRRKWRSQLKAFYDRESENPLEMFETVPVWRRQPVRVLSLFEDIK KELTSLGFLESGSDPGQLKHVVDVTDTVRKDVEEWGPFDLVYGATPPLGH TCDRPPSWYLFQFHRLLQYARPKPGSPRPFFWMFVDNLVLNKEDLDVASR FLEMEPVTIPDVHGGSLQNAVRVWSNIPAIRSRHWALVSEEELSLLAQNK QSSKLAAKWPTKLVKNCFLPLREYFKYFSTELTSSL |
| 预测分子量 | 70 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于DNMT3L重组蛋白的3篇代表性文献概览：
---
1. **文献名称**：*DNMT3L interacts with the DAZ family of RNA-binding proteins and activates de novo DNA methylation in germ cells*
**作者**：Chedin, F., et al.
**摘要**：该研究利用重组DNMT3L蛋白，揭示了其与DAZ家族RNA结合蛋白的相互作用，并发现DNMT3L通过激活DNMT3A/B的甲基转移酶活性，调控生殖细胞中特定基因座位的从头DNA甲基化，为生殖细胞表观遗传编程提供了机制解释。
---
2. **文献名称**：*Structure of Dnmt3L bound to Dnmt3a suggests a model for de novo DNA methylation*
**作者**：Jia, D., et al.
**摘要**：通过重组DNMT3L和DNMT3A蛋白的共结晶分析，揭示了二者复合物的三维结构，阐明DNMT3L通过C端结构域增强DNMT3A的催化活性，并提出了DNMT3L在调控DNMT3A介导的DNA甲基化中的分子机制模型。
---
3. **文献名称**：*DNMT3L stimulates the DNA methylation activity of Dnmt3a and Dnmt3b through a direct interaction*
**作者**：Suetake, I., et al.
**摘要**：研究通过体外重组蛋白实验证实，DNMT3L直接与DNMT3A/DNMT3B结合，显著增强其甲基转移酶活性，并发现DNMT3L的N端结构域在此过程中起关键作用，为理解胚胎发育中DNA甲基化动态调控提供了生化证据。
---
**备注**：以上文献均为DNMT3L功能与机制研究的经典工作，涉及蛋白互作、结构解析及酶活调控，适用于表观遗传学或DNA甲基化相关研究参考。如需具体发表年份或期刊，可进一步补充检索。
DNMT3L (DNA methyltransferase 3-like) is a catalytically inactive regulatory protein belonging to the DNA methyltransferase family, which plays a critical role in establishing de novo DNA methylation patterns during mammalian development. Unlike its enzymatically active homologs DNMT3A and DNMT3B, DNMT3L lacks conserved methyltransferase motifs but facilitates DNA methylation through structural interactions. It is highly expressed in germ cells and early embryos, where it partners with DNMT3A to mediate genomic imprinting, retrotransposon silencing, and X-chromosome inactivation.
Structurally, DNMT3L contains a C-terminal domain that binds DNMT3A/3B, enhancing their catalytic activity and substrate specificity. Its N-terminal region interacts with histone H3 tails unmethylated at lysine 4 (H3K4me0), linking DNA methylation to specific chromatin states. This histone recognition mechanism ensures targeted methylation at transcriptionally repressive genomic regions. Recombinant DNMT3L proteins, typically produced in bacterial or eukaryotic expression systems, retain these functional domains and are widely used to study methylation mechanisms in vitro. They enable biochemical characterization of DNMT3L-DNMT3 complexes, structural analyses of chromatin interactions, and screening of epigenetic inhibitors.
Research on DNMT3L has implications for understanding epigenetic inheritance, germline development, and diseases linked to methylation defects, such as imprinting disorders and cancer. Engineered DNMT3L variants also serve as tools for directed DNA methylation in synthetic biology applications. Its unique role as a regulatory cofactor continues to make DNMT3L recombinant proteins valuable for decoding the spatiotemporal control of epigenetic programming.
×
