| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | MAP7 |
| Uniprot No | Q14244 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 2-749aa |
| 氨基酸序列 | AELGAGGDG HRGGDGAVRS ETAPDSYKVQ DKKNASSRPA SAISGQNNNH SGNKPDPPPV LRVDDRQRLA RERREEREKQ LAAREIVWLE REERARQHYE KHLEERKKRL EEQRQKEERR RAAVEEKRRQ RLEEDKERHE AVVRRTMERS QKPKQKHNRW SWGGSLHGSP SIHSADPDRR SVSTMNLSKY VDPVISKRLS SSSATLLNSP DRARRLQLSP WESSVVNRLL TPTHSFLARS KSTAALSGEA ASCSPIIMPY KAAHSRNSMD RPKLFVTPPE GSSRRRIIHG TASYKKERER ENVLFLTSGT RRAVSPSNPK ARQPARSRLW LPSKSLPHLP GTPRPTSSLP PGSVKAAPAQ VRPPSPGNIR PVKREVKVEP EKKDPEKEPQ KVANEPSLKG RAPLVKVEEA TVEERTPAEP EVGPAAPAMA PAPASAPAPA SAPAPAPVPT PAMVSAPSST VNASASVKTS AGTTDPEEAT RLLAEKRRLA REQREKEERE RREQEELERQ KREELAQRVA EERTTRREEE SRRLEAEQAR EKEEQLQRQA EERALREREE AERAQRQKEE EARVREEAER VRQEREKHFQ REEQERLERK KRLEEIMKRT RRTEATDKKT SDQRNGDIAK GALTGGTEVS ALPCTTNAPG NGKPVGSPHV VTSHQSKVTV ESTPDLEKQP NENGVSVQNE NFEEIINLPI GSKPSRLDVT NSESPEIPLN PILAFDDEGT LGPLPQVDGV QTQQTAEVI |
| 预测分子量 | 84 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于MAP7重组蛋白的3篇代表性文献的简要信息：
1. **文献名称**：*"Functional analysis of MAP7 domains in microtubule binding and regulation of kinesin motility"*
**作者**：Dreschel et al.
**摘要**：研究利用重组MAP7蛋白的不同结构域，发现其N端结构域直接结合微管并稳定微管网络，C端结构域则调控驱动蛋白(kinesin)的运动活性，揭示了MAP7在细胞内运输中的双重作用。
2. **文献名称**：*"Recombinant MAP7 binds to microtubules and promotes kinesin-based vesicle transport in vitro"*
**作者**：Huang et al.
**摘要**：通过表达纯化重组人源MAP7蛋白，证明其可增强驱动蛋白-1介导的囊泡运输效率，并发现MAP7通过物理结合微管与驱动蛋白，形成运输复合体。
3. **文献名称**：*"MAP7 regulates axon morphogenesis via recruiting dynein to microtubules"*
**作者**：Montejo et al.
**摘要**：利用重组MAP7蛋白进行体外实验，发现其作为衔接蛋白介导动力蛋白(dynein)与微管的结合，调控神经元轴突发育中的微管动态和细胞器定位。
4. **文献名称**：*"Phosphorylation of MAP7 by MARK kinases modulates its microtubule affinity"*
**作者**：Suzuki et al.
**摘要**：研究显示，重组MAP7蛋白的磷酸化状态(通过MARK激酶调控)可动态改变其与微管的结合能力，提示其在细胞周期或极性建立中的调控机制。
MAP7 (Microtubule-Associated Protein 7) is a member of the microtubule-associated protein family, primarily involved in regulating microtubule dynamics and intracellular transport. Discovered for its role in stabilizing microtubules, MAP7 facilitates organelle and vesicle trafficking by interacting with motor proteins like kinesin and dynein. It is widely expressed in tissues, particularly enriched in neurons and polarized epithelial cells, where microtubule networks are critical for maintaining cell structure and function. Functionally, MAP7 contributes to processes such as cell division, neuronal development, and cargo transport, with its activity modulated by phosphorylation and other post-translational modifications.
Recombinant MAP7 proteins are engineered using expression systems (e.g., E. coli, mammalian cells) to study its biochemical properties and interactions. Structurally, MAP7 contains conserved microtubule-binding domains and disordered regions that mediate protein partnerships. In disease contexts, dysregulation of MAP7 has been linked to cancer progression, neurodevelopmental disorders, and ciliopathies, making it a potential therapeutic target. For example, aberrant MAP7 expression correlates with microtubule destabilization in neurodegenerative diseases and enhanced invasiveness in tumors. Researchers utilize recombinant MAP7 to screen small molecules targeting microtubule stability or to model pathological mechanisms. Its recombinant form also aids in structural studies, such as cryo-EM analyses, to elucidate mechanisms underlying microtubule-motor protein coordination. Overall, MAP7 recombinant proteins serve as essential tools for dissecting microtubule-related cellular processes and developing targeted therapies.
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