| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | ACSL3 |
| Uniprot No | O95573 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 42-720aa |
| 氨基酸序列 | YFFSESRQEKSNRIKAKPVNSKPDSAYRSVNSLDGLASVLYPGCDTLDKVFTYAKNKFKNKRLLGTREVLNEEDEVQPNGKIFKKVILGQYNWLSYEDVFVRAFNFGNGLQMLGQKPKTNIAIFCETRAEWMIAAQACFMYNFQLVTLYATLGGPAIVHALNETEVTNIITSKELLQTKLKDIVSLVPRLRHIITVDGKPPTWSEFPKGIIVHTMAAVEALGAKASMENQPHSKPLPSDIAVIMYTSGSTGLPKGVMISHSNIIAGITGMAERIPELGEEDVYIGYLPLAHVLELSAELVCLSHGCRIGYSSPQTLADQSSKIKKGSKGDTSMLKPTLMAAVPEIMDRIYKNVMNKVSEMSSFQRNLFILAYNYKMEQISKGRNTPLCDSFVFRKVRSLLGGNIRLLLCGGAPLSATTQRFMNICFCCPVGQGYGLTESAGAGTISEVWDYNTGRVGAPLVCCEIKLKNWEEGGYFNTDKPHPRGEILIGGQSVTMGYYKNEAKTKADFFEDENGQRWLCTGDIGEFEPDGCLKIIDRKKDLVKLQAGEYVSLGKVEAALKNLPLVDNICAYANSYHSYVIGFVVPNQKELTELARKKGLKGTWEELCNSCEMENEVLKVLSEAAISASLEKFEIPVKIRLSPEPWTPETGLVTDAFKLKRKELKTHYQADIERMYGRK |
| 预测分子量 | 76.9 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于ACSL3重组蛋白的3篇参考文献概览：
1. **文献名称**：*"Functional characterization of recombinant human acyl-CoA synthetase 3 (ACSL3) in lipid metabolism"*
**作者**：Li X, et al.
**摘要**：研究通过重组表达人源ACSL3蛋白，分析其对不同脂肪酸底物的催化活性，发现ACSL3优先激活长链单不饱和脂肪酸，并揭示其在脂滴形成中的调控作用。
2. **文献名称**：*"Structural insights into ACSL3 catalysis and membrane association via cryo-EM"*
**作者**：Zhang Y, et al.
**摘要**：利用冷冻电镜解析重组ACSL3蛋白的立体结构，阐明其底物结合口袋的构象变化及与细胞膜相互作用的分子机制。
3. **文献名称**：*"ACSL3 promotes prostate cancer metastasis by mediating fatty acid oxidation"*
**作者**：Wang H, et al.
**摘要**：通过重组ACSL3蛋白体外功能实验，证实其通过增强脂肪酸氧化(FAO)促进前列腺癌细胞侵袭转移，为靶向ACSL3的癌症治疗提供依据。
注：以上文献为示例性概括，实际引用需核对具体文献来源及细节。
ACSL3 (Acyl-CoA Synthetase Long-Chain Family Member 3) is a key enzyme in lipid metabolism that catalyzes the activation of long-chain fatty acids into acyl-CoA esters, a critical step for fatty acid oxidation, membrane synthesis, and lipid-mediated signaling. It belongs to the acyl-CoA synthetase family, which plays a central role in cellular energy homeostasis. Dysregulation of ACSL3 has been linked to metabolic disorders, cancer progression (e.g., lung and prostate cancers), and neurodegenerative diseases, making it a target for therapeutic research.
Recombinant ACSL3 protein is produced using biotechnological methods, typically through cloning the ACSL3 gene into expression vectors (e.g., bacterial, mammalian, or insect cell systems) followed by purification via affinity chromatography. This engineered protein retains the enzymatic activity of native ACSL3. enabling in vitro studies to dissect its biochemical mechanisms, substrate specificity, and interactions with inhibitors or modulators. Researchers utilize recombinant ACSL3 to investigate its role in lipid droplet formation, cancer cell proliferation, and drug resistance. It also serves as a tool for high-throughput screening of potential therapeutics targeting lipid metabolism pathways. The availability of purified recombinant ACSL3 accelerates structural studies (e.g., crystallography) and the development of diagnostic or therapeutic agents for diseases associated with lipid dysregulation.
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