| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | NEFM |
| Uniprot No | P07197 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 487-740aa |
| 氨基酸序列 | EKKEAAEEKEEEPEAEEEEVAAKKSPVKATAPEVKEEEGEKEEEEGQEEEEEEDEGAKSDQAEEGGSEKEGSSEKEEGEQEEGETEAEAEGEEAEAKEEKKVEEKSEEVATKEELVADAKVEKPEKAKSPVPKSPVEEKGKSPVPKSPVEEKGKSPVPKSPVEEKGKSPVPKSPVEEKGKSPVSKSPVEEKAKSPVPKSPVEEAKSKAEVGKGEQKEEEEKEVKEAPKEEKVEKKEEKPKDVPEKKKAESPVKE |
| 预测分子量 | 29.3 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是几篇模拟的关于NEFM重组蛋白的参考文献示例(请注意，这些为虚构内容，实际文献需通过学术数据库查询)：
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1. **标题**：*Expression and Purification of Recombinant NEFM in E. coli for Neurofilament Assembly Studies*
**作者**：Smith, J., et al.
**摘要**：本研究报道了在大肠杆菌系统中高效表达人源NEFM重组蛋白的优化方法，通过亲和层析纯化获得高纯度蛋白，并验证其在体外神经丝纤维自组装中的功能。
2. **标题**：*Phosphorylation Dynamics of Recombinant NEFM and Its Role in Axonal Stability*
**作者**：Lee, H., et al.
**摘要**：利用重组NEFM蛋白探究其磷酸化修饰对神经丝稳定性的影响，发现特定激酶(如CDK5)介导的磷酸化可调控神经细胞骨架的完整性。
3. **标题**：*NEFM as a Biomarker in ALS: Development of a Recombinant Protein-Based ELISA Assay*
**作者**：Garcia, R., et al.
**摘要**：基于重组NEFM蛋白开发了一种高灵敏度ELISA检测方法，用于定量ALS患者脑脊液中的神经丝蛋白水平，证实其与疾病进展的相关性。
4. **标题**：*Structural Characterization of Recombinant NEFM Using Cryo-Electron Microscopy*
**作者**：Wang, Y., et al.
**摘要**：通过冷冻电镜解析了重组NEFM蛋白的分子结构，揭示了其与神经丝轻链(NEFL)相互作用的分子机制。
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**建议**：如需真实文献，可在PubMed或Google Scholar中搜索关键词：
`recombinant NEFM protein`, `neurofilament medium subunit expression`, `NEFM phosphorylation`, `NEFM biomarker neurodegenerative`。
**Background of NEFM Recombinant Protein**
Neurofilament medium polypeptide (NEFM) is a critical component of neuronal intermediate filaments, belonging to the neurofilament triplet protein family alongside NEFL (light) and NEFH (heavy). It plays a structural role in maintaining axon caliber and mechanical stability, influencing nerve conduction velocity. Neurofilaments, composed of these subunits, form a dynamic cytoskeletal network essential for neuronal integrity, particularly in long axons of motor and sensory neurons.
Recombinant NEFM protein is engineered using molecular cloning techniques, typically expressed in *E. coli* or mammalian cell systems to ensure proper post-translational modifications. Its production enables detailed study of neurofilament assembly, axonal transport, and interactions with other cytoskeletal proteins. Dysregulation of NEFM is implicated in neurodegenerative diseases (e.g., ALS, Charcot-Marie-Tooth) and neurotrauma, where neurofilament accumulation or phosphorylation abnormalities contribute to pathology.
Research applications include *in vitro* modeling of neurodegeneration, biomarker development (e.g., detecting neurofilament light/medium/heavy chains in cerebrospinal fluid or blood), and screening therapeutic agents targeting neurofilament-related dysfunction. Recombinant NEFM also aids in generating antibodies for diagnostic tools and elucidating mechanisms of axonal injury. Its study bridges gaps between structural neurobiology and clinical neurology, offering insights into both disease mechanisms and potential interventions.
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