| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | RPL28 |
| Uniprot No | P46779 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 2-137aa |
| 氨基酸序列 | SAHLQWMVVRNCSSFLIKRNKQTYSTEPNNLKARNSFRYNGLIHRKTVGVEPAADGKGVVVVIKRRSGQRKPATSYVRTTINKNARATLSSIRHMIRKNKYRPDLRMAAIRRASAILRSQKPVMVKRKRTRPTKSS |
| 预测分子量 | 42.6 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于RPL28重组蛋白的3篇代表性文献示例(注：以下为模拟内容，具体文献需根据实际数据库查询)：
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1. **文献名称**: *Structural insights into the role of RPL28 in ribosome assembly*
**作者**: Zhang Y, et al.
**摘要**: 本研究解析了重组人源RPL28蛋白的晶体结构，揭示了其与核糖体RNA(rRNA)结合的分子机制，表明RPL28在核糖体大亚基组装过程中可能通过特定结构域介导构象变化。
2. **文献名称**: *RPL28 knockdown impairs protein synthesis and cell proliferation in cancer cells*
**作者**: Li X, et al.
**摘要**: 通过重组RPL28蛋白回补实验，发现抑制内源性RPL28会导致癌细胞蛋白质合成速率下降和细胞周期阻滞，提示RPL28在肿瘤生长中具有关键调控作用。
3. **文献名称**: *RPL28 interacts with MYC oncoprotein to regulate target gene translation*
**作者**: Wang H, et al.
**摘要**: 利用重组RPL28进行免疫共沉淀实验，发现RPL28与MYC蛋白直接结合，并通过核糖体招募机制增强特定致癌mRNA的翻译效率，为癌症治疗提供了潜在靶点。
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实际文献需通过PubMed、Web of Science或Google Scholar以关键词“RPL28 recombinant protein”或“RPL28 function”检索获取最新数据。
**Background of RPL28 Recombinant Protein**
RPL28 (Ribosomal Protein L28) is a component of the 60S ribosomal subunit, playing a critical role in protein biosynthesis by facilitating mRNA decoding and peptide bond formation. As part of the ribosome, RPL28 contributes to structural stability and ensures translational fidelity. Beyond its canonical role, RPL28 has been implicated in extraribosomal functions, including cell proliferation, apoptosis, and DNA repair, with dysregulation linked to cancers and developmental disorders.
Recombinant RPL28 protein is engineered via molecular cloning, where the RPL28 gene is inserted into expression vectors (e.g., bacterial, yeast, or mammalian systems) to enable large-scale production. This approach ensures high purity and avoids contaminants from native sources. The recombinant protein typically retains post-translational modifications when expressed in eukaryotic systems, preserving functional relevance.
Studies utilizing RPL28 recombinant protein focus on elucidating its structural interactions within the ribosome and its role in stress responses or disease mechanisms. For instance, it aids in mapping binding partners via pull-down assays or characterizing mutations affecting ribosome assembly in genetic disorders. In cancer research, RPL28 overexpression has been observed in certain tumors, suggesting its potential as a biomarker or therapeutic target.
Additionally, recombinant RPL28 serves as an antigen for antibody development, enabling precise detection in cellular localization or expression profiling. Its application in structural biology (e.g., cryo-EM or X-ray crystallography) provides insights into ribosome dynamics and antibiotic interactions.
Overall, RPL28 recombinant protein is a vital tool for dissecting ribosomal biology, disease pathways, and translational regulation, bridging fundamental research with clinical applications.
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