| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | PMEL |
| Uniprot No | P40967 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-661aa |
| 氨基酸序列 | MDLVLKRCLLHLAVIGALLAVGATKVPRNQDWLGVSRQLRTKAWNRQLYP EWTEAQRLDCWRGGQVSLKVSNDGPTLIGANASFSIALNFPGSQKVLPDG QVIWVNNTIINGSQVWGGQPVYPQETDDACIFPDGGPCPSGSWSQKRSFV YVWKTWGQYWQVLGGPVSGLSIGTGRAMLGTHTMEVTVYHRRGSRSYVPL AHSSSAFTITDQVPFSVSVSQLRALDGGNKHFLRNQPLTFALQLHDPSGY LAEADLSYTWDFGDSSGTLISRALVVTHTYLEPGPVTAQVVLQAAIPLTS CGSSPVPGTTDGHRPTAEAPNTTAGQVPTTEVVGTTPGQAPTAEPSGTTS VQVPTTEVISTAPVQMPTAESTGMTPEKVPVSEVMGTTLAEMSTPEATGM TPAEVSIVVLSGTTAAQVTTTEWVETTARELPIPEPEGPDASSIMSTESI TGSLGPLLDGTATLRLVKRQVPLDCVLYRYGSFSVTLDIVQGIESAEILQ AVPSGEGDAFELTVSCQGGLPKEACMEISSPGCQPPAQRLCQPVLPSPAC QLVLHQILKGGSGTYCLNVSLADTNSLAVVSTQLIMPGQEAGLGQVPLIV GILLVLMAVVLASLIYRRRLMKQDFSVPQLPHSSSHWLRLPRIFCSCPIG ENSPLLSGQQV |
| 预测分子量 | 97 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于PMEL重组蛋白的3篇参考文献及其摘要概括：
1. **"Recombinant PMEL17 as a tool for studying melanosome biogenesis"**
- **作者**: Theos AC, et al.
- **摘要**: 研究利用重组PMEL17蛋白解析其在黑色素体形成中的作用，通过体外实验证明其能够自发聚集成淀粉样纤维结构，为黑色素沉积提供支架。
2. **"Expression and purification of functional PMEL for immunological studies"**
- **作者**: Berson JF, et al.
- **摘要**: 开发了一种高效表达和纯化重组PMEL蛋白的方法，并验证其在免疫检测中的应用潜力，为黑色素瘤相关抗体研究提供了工具。
3. **"PMEL amyloid fibril formation: Key determinants in the repeat domain"**
- **作者**: Watt B, et al.
- **摘要**: 通过重组PMEL蛋白的重复结构域突变分析，揭示了其淀粉样纤维形成的分子机制，为病理性淀粉样沉积研究提供了新视角。
*注：文献标题与作者为示例性内容，实际引用时需以具体数据库(如PubMed)检索结果为准。建议补充关键词(如PMEL/Pmel17、重组表达、黑色素瘤)进一步筛选应用场景相关研究。*
**Background of PMEL Recombinant Proteins**
PMEL (Premelanosome Protein), also known as SILV or gp100. is a melanocyte-specific glycoprotein critical for melanin synthesis. Naturally expressed in pigment-producing cells, PMEL plays a structural role in forming amyloid fibrils within melanosomes, organelles responsible for melanin production and storage. These fibrils serve as scaffolds for melanin deposition, ensuring efficient pigment synthesis and distribution in tissues like skin, hair, and eyes.
Recombinant PMEL proteins are engineered *in vitro* to replicate functional domains of the native protein, such as the N-terminal PKD domain or the amyloidogenic repeat (RPT) domain. These recombinant forms are typically produced in heterologous expression systems (e.g., *E. coli*, mammalian cells) and purified for research or therapeutic applications. Key interest in PMEL stems from its dual role in melanogenesis and its amyloidogenic properties, which are non-toxic compared to pathological amyloids (e.g., Aβ in Alzheimer’s).
In biomedical research, recombinant PMEL is used to study melanosome biogenesis, pigment-related disorders (e.g., melanoma, vitiligo), and amyloid formation mechanisms. Its immunogenic epitopes, particularly the gp100 antigen, have been exploited in cancer immunotherapy, such as melanoma vaccines, to stimulate T-cell responses. Additionally, PMEL’s self-assembling fibrils inspire biomaterial design for drug delivery or tissue engineering.
Overall, PMEL recombinant proteins serve as versatile tools bridging cell biology, immunology, and nanotechnology, offering insights into both physiological processes and disease mechanisms.
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