| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | SLC41A2 |
| Uniprot No | Q96JW4 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-162aa |
| 氨基酸序列 | MTNSKGRSITDKTSGGPSSGGGFVDWTLRLNTIQSDKFLNLLLSMVPVIYQKNQEDRHKKANGIWQDGLSTAVQTFSNRSEQHMEYHSFSEQSFHANNGHASSSCSQKYDDYANYNYCDGRETSETTAMLQDEDISSDGDEDAIVEVTPKLPKESSGIMALQ |
| 预测分子量 | 22.0 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是基于SLC41A2重组蛋白研究领域模拟的参考文献示例(非真实文献，仅供格式参考)：
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1. **文献名称**: *"Functional characterization of recombinant human SLC41A2 as a cellular magnesium transporter"*
**作者**: Smith J, et al.
**摘要**: 本研究通过在大肠杆菌和哺乳动物细胞中重组表达SLC41A2蛋白，证实其具有镁离子转运活性，并依赖跨膜电化学梯度。实验表明SLC41A2的缺失会导致细胞内镁稳态失衡，提示其在生理和病理中的潜在作用。
2. **文献名称**: *"Crystal structure of the SLC41A2 cation transporter reveals key residues for ion selectivity"*
**作者**: Zhang Y, et al.
**摘要**: 通过冷冻电镜解析了重组人源SLC41A2蛋白的高分辨率结构，揭示了其跨膜结构域中决定二价阳离子(Mg²⁺/Fe²⁺)选择性的关键氨基酸残基，为靶向该蛋白的药物设计提供结构基础。
3. **文献名称**: *"SLC41A2 knockdown exacerbates renal fibrosis via dysregulation of magnesium-dependent cellular pathways"*
**作者**: Lee H, et al.
**摘要**: 利用重组SLC41A2蛋白模型，发现其在肾脏细胞中通过调控镁离子水平影响TGF-β信号通路，缺失SLC41A2会加剧纤维化进程，提示其作为慢性肾病治疗靶点的潜力。
4. **文献名称**: *"High-throughput screening identifies novel inhibitors of SLC41A2-mediated magnesium transport"*
**作者**: Gupta R, et al.
**摘要**: 通过基于重组SLC41A2蛋白的体外转运实验筛选小分子化合物库，发现两类可逆性抑制剂，为研究镁代谢疾病及开发调节剂提供工具分子。
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**注意**：以上文献为模拟生成，实际研究中请通过 **PubMed/Google Scholar** 等平台检索关键词(如 "SLC41A2 recombinant" "SLC41A2 magnesium transporter")获取真实文献。近期研究可能涉及该蛋白在神经系统或癌症中的功能探索。
**Background of SLC41A2 Recombinant Protein**
SLC41A2 (Solute Carrier Family 41 Member 2) is a magnesium transporter belonging to the SLC41 family, which plays a critical role in cellular magnesium homeostasis. This transmembrane protein facilitates Mg²⁺ transport across plasma membranes or intracellular organelles, regulating processes like enzymatic activity, energy metabolism, and nucleic acid synthesis. SLC41A2 is ubiquitously expressed, with notable presence in tissues such as the kidney, intestine, and brain, where magnesium balance is vital for physiological functions.
Recombinant SLC41A2 protein is engineered for *in vitro* studies to elucidate its structural and functional characteristics. Researchers produce it using expression systems (e.g., mammalian, insect, or bacterial cells) to obtain purified, bioactive protein. This enables investigations into its transport mechanisms, substrate specificity, and interaction with regulatory factors. Dysregulation of SLC41A2 has been implicated in pathologies like hypertension, neurodegenerative disorders, and cancer, highlighting its therapeutic relevance.
Studies using recombinant SLC41A2 have advanced understanding of its role in magnesium-dependent signaling pathways and potential crosstalk with other ion transporters. Challenges remain in resolving its 3D structure and precise regulatory networks. Nonetheless, recombinant SLC41A2 serves as a crucial tool for drug discovery, aiming to modulate magnesium transport in disease contexts. Ongoing research focuses on its genetic variants, tissue-specific functions, and contribution to systemic magnesium balance, offering insights into novel diagnostic or therapeutic strategies.
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