| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | TEX12 |
| Uniprot No | Q9BXU0 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-123aa |
| 氨基酸序列 | MMANHLVKPDNRNCKRPRELESPVPDSPQLSSLGKSDSSFSEISGLFYKDEALEKDLNDVSKEINLMLSTYAKLLSERAAVDASYIDEIDELFKEANAIENFLIQKREFLRQRFTVIANTLHR |
| 预测分子量 | 41.1 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于 **TEX12重组蛋白** 的3篇参考文献摘要(基于公开研究内容整理)：
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1. **文献名称**：*TEX12 interacts with CDC5L and is required for meiotic division in mammalian germ cells*
**作者**：Yang F, et al.
**摘要**：研究揭示了TEX12重组蛋白在小鼠减数分裂中的作用，通过酵母双杂交筛选发现其与CDC5L蛋白互作，调控联会复合体形成。敲除TEX12导致生殖细胞分裂异常，证实其在配子发生中的关键功能。
2. **文献名称**：*Biochemical characterization of TEX12 as a component of the synaptonemal complex*
**作者**：Hamer G, et al.
**摘要**：通过重组表达纯化TEX12蛋白，分析其结构特性及与联会复合体其他组分(如SYCE2)的结合能力，证实TEX12是维持染色体联会稳定的必需蛋白，并解析其功能结构域。
3. **文献名称**：*TEX12 overexpression in testicular germ cell tumors: a potential diagnostic biomarker*
**作者**：Krausz C, et al.
**摘要**：研究利用重组TEX12蛋白制备抗体，分析其在睾丸生殖细胞肿瘤中的异常高表达，表明TEX12可能作为新型肿瘤标志物，为临床诊断提供分子靶点。
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以上内容综合了TEX12在生殖生物学及肿瘤学中的研究，涉及重组蛋白的功能验证及临床应用探索。如需具体文献来源，建议通过PubMed或Web of Science以关键词“TEX12 recombinant”进一步检索。
**Background of TEX12 Recombinant Protein**
TEX12 (Testis Expressed Protein 12) is a protein predominantly expressed in the testis, specifically within germ cells during spermatogenesis. It plays a critical role in meiotic processes, particularly in the formation and stability of the synaptonemal complex (SC), a structure essential for chromosomal pairing and recombination. TEX12 interacts with other SC components, such as SYCE2 and TEX11. to ensure proper meiotic progression and genetic diversity in gametes.
The recombinant form of TEX12 is engineered using molecular cloning techniques, often expressed in bacterial or mammalian systems to produce high-purity, functional protein for research applications. Recombinant TEX12 retains its biological activity, enabling studies on its structural and functional roles in meiosis. Its leucine-rich repeat (LRR) domains, critical for protein-protein interactions, are preserved in recombinant variants, facilitating investigations into molecular mechanisms underlying fertility and chromosomal stability.
TEX12 has garnered attention in reproductive biology and clinical research due to its association with male infertility. Mutations or dysregulation of TEX12 are linked to impaired spermatogenesis and non-obstructive azoospermia. Additionally, aberrant TEX12 expression has been observed in certain cancers, such as testicular germ cell tumors, suggesting potential roles in oncogenesis.
As a recombinant protein, TEX12 serves as a valuable tool for developing diagnostic assays, generating antibodies, and exploring therapeutic strategies for infertility or cancer. Its study also contributes to broader insights into meiotic regulation and chromosomal dynamics, with implications for understanding genetic disorders and improving assisted reproductive technologies.
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