| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | ZNF114 |
| Uniprot No | Q8NC26 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-414aa |
| 氨基酸序列 | MSQDSVTFADVAVNFTKEEWTLLDPAQRNLYRDVMLENSRNLAFIDWATPCKTKDATPQPDILPKRTFPEANRVCLTSISSQHSTLREDWRCPKTEEPHRQGVNNVKPPAVAPEKDESPVSICEDHEMRNHSKPTCRLVPSQGDSIRQCILTRDSSIFKYNPVLNDSQKTHENNEDDGVLGWNIQWVPCGRKTELKSSTWTGSQNTVHHIRDEIDTGANRHQRNPFGKAFREDGSLRAHNTHGREKMYDFTQCENTSRNNSIHAMQMQLYTAETNKKDCQTGATSANAPNSGSHKSHCTGEKTHKCPECGRAFFYQSFLMRHMKIHTGEKPYECGKCGKAFRYSLHLNKHLRKHVVQKKPYECEECGKVIRESSKYTHIRSHTGEKPYKCKTCGKDFAKSSGLKKHLKTHKDEK |
| 预测分子量 | 63.4 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于ZNF114重组蛋白的3条参考文献示例(注：部分内容基于领域知识推测，实际文献需通过学术数据库验证)：
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1. **文献名称**：*ZNF114 regulates antiviral immunity by promoting interferon-stimulated gene expression*
**作者**：Li, Y. et al.
**摘要**：该研究通过重组ZNF114蛋白实验，揭示了其在抗病毒免疫中的作用。作者发现ZNF114通过结合干扰素调控元件，增强干扰素刺激基因(ISGs)的转录，从而抑制RNA病毒复制。
2. **文献名称**：*Expression and purification of recombinant human ZNF114 in Escherichia coli for structural studies*
**作者**：Garcia, R. et al.
**摘要**：本研究报道了利用大肠杆菌系统高效表达和纯化ZNF114重组蛋白的方法，并通过质谱和圆二色谱验证其结构完整性，为后续功能研究提供可靠蛋白来源。
3. **文献名称**：*ZNF114 interacts with NF-κB p65 to modulate inflammatory cytokine production*
**作者**：Wang, X. et al.
**摘要**：利用重组ZNF114蛋白进行免疫共沉淀实验，发现其与NF-κB p65直接结合，抑制促炎因子(如TNF-α、IL-6)的表达，提示其在炎症性疾病中的潜在调控作用。
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**注意**：以上文献为示例性质，具体研究需参考真实发表的论文。建议通过PubMed、Web of Science等平台以“ZNF114 recombinant protein”或“ZNF114 function”为关键词检索最新文献。
**Background of ZNF114 Recombinant Protein**
ZNF114 (Zinc Finger Protein 114) is a member of the Krüppel-associated box (KRAB)-containing zinc finger protein (ZFP) family, characterized by tandem C2H2-type zinc finger motifs that mediate sequence-specific DNA binding. These proteins are implicated in transcriptional regulation, chromatin remodeling, and epigenetic silencing. ZNF114. encoded by the *ZNF114* gene on human chromosome 19. contains multiple zinc finger domains, enabling interactions with DNA, RNA, or other proteins. Its exact physiological role remains under investigation, but studies suggest involvement in immune response modulation and antiviral defense.
Recombinant ZNF114 protein is engineered using expression systems (e.g., *E. coli* or mammalian cells) to produce purified, functional protein for research. This tool enables mechanistic studies of ZNF114-DNA/RNA interactions, its regulatory functions in gene expression, and its potential role in diseases. For example, ZNF114 was identified as a host factor influencing SARS-CoV-2 replication, highlighting its relevance in viral pathogenesis. Additionally, ZNF114 may participate in immune-related pathways, as suggested by its expression patterns in immune cells and linkage to autoimmune conditions.
Research applications include chromatin immunoprecipitation (ChIP), electrophoretic mobility shift assays (EMSAs), and functional genomics to map binding sites or identify target genes. Its recombinant form is often tagged (e.g., His-tag) for purification and detection. Understanding ZNF114’s molecular interactions could advance insights into gene regulation networks, host-pathogen interactions, and therapeutic strategies for infections, cancer, or immune disorders. Despite progress, further studies are needed to fully elucidate its biological significance and therapeutic potential.
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