| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | Caprin2 |
| Uniprot No | Q6IMN6 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-1127aa |
| 氨基酸序列 | MEVQVSQASLGFELTSVEKSLREWSRLSREVIAWLCPSSPNFILNFPPPPSASSVSMVQLFSSPFGYQSPSGHSEEEREGNMKSAKPQVNHSQHGESQRALSPLQSTLSSAASPSQAYETYIENGLICLKHKIRNIEKKKLKLEDYKDRLKSGEHLNPDQLEAVEKYEEVLHNLEFAKELQKTFSGLSLDLLKAQKKAQRREHMLKLEAEKKKLRTILQVQYVLQNLTQEHVQKDFKGGLNGAVYLPSKELDYLIKFSKLTCPERNESLSVEDQMEQSSLYFWDLLEGSEKAVVGTTYKHLKDLLSKLLNSGYFESIPVPKNAKEKEVPLEEEMLIQSEKKTQLSKTESVKESESLMEFAQPEIQPQEFLNRRYMTEVDYSNKQGEEQPWEADYARKPNLPKRWDMLTEPDGQEKKQESFKSWEASGKHQEVSKPAVSLEQRKQDTSKLRSTLPEEQKKQEISKSKPSPSQWKQDTPKSKAGYVQEEQKKQETPKLWPVQLQKEQDPKKQTPKSWTPSMQSEQNTTKSWTTPMCEEQDSKQPETPKSWENNVESQKHSLTSQSQISPKSWGVATASLIPNDQLLPRKLNTEPKDVPKPVHQPVGSSSTLPKDPVLRKEKLQDLMTQIQGTCNFMQESVLDFDKPSSAIPTSQPPSATPGSPVASKEQNLSSQSDFLQEPLQATSSPVTCSSNACLVTTDQASSGSETEFMTSETPEAAIPPGKQPSSLASPNPPMAKGSEQGFQSPPASSSSVTINTAPFQAMQTVFNVNAPLPPRKEQEIKESPYSPGYNQSFTTASTQTPPQCQLPSIHVEQTVHSQETAANYHPDGTIQVSNGSLAFYPAQTNVFPRPTQPFVNSRGSVRGCTRGGRLITNSYRSPGGYKGFDTYRGLPSISNGNYSQLQFQAREYSGAPYSQRDNFQQCYKRGGTSGGPRANSRAGWSDSSQVSSPERDNETFNSGDSGQGDSRSMTPVDVPVTNPAATILPVHVYPLPQQMRVAFSAARTSNLAPGTLDQPIVFDLLLNNLGETFDLQLGRFNCPVNGTYVFIFHMLKLAVNVPLYVNLMKNEEVLVSAYANDGAPDHETASNHAILQLFQGDQIWLRLHRGAIYGSSWKYSTFSGYLLYQD |
| 预测分子量 | 125,9 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于Caprin2重组蛋白研究的示例参考文献(内容基于常见研究方向，文献信息为假设性示例)：
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1. **文献名称**: *"Expression and Purification of Recombinant Caprin2 in E. coli for Functional Studies"*
**作者**: Li, X., et al. (2018)
**摘要**: 本研究报道了通过原核表达系统(大肠杆菌)高效表达并纯化重组Caprin2蛋白的优化方法。通过His标签亲和层析获得高纯度蛋白，并验证其与β-catenin的体外相互作用，为后续功能研究提供材料基础。
2. **文献名称**: *"Caprin2 Regulates Wnt/β-Catenin Signaling by Stabilizing LRP6 in Hepatocellular Carcinoma"*
**作者**: Wang, Y., et al. (2019)
**摘要**: 研究利用重组Caprin2蛋白进行体外实验，发现其通过结合LRP6受体增强Wnt/β-catenin信号通路活性，促进肝癌细胞增殖。重组蛋白的过表达实验为机制验证提供了关键证据。
3. **文献名称**: *"Structural Insights into Caprin2-Mediated mRNA Transport Complex Assembly"*
**作者**: Zhang, R., et al. (2020)
**摘要**: 通过重组Caprin2蛋白的晶体结构解析，揭示了其N端结构域与RNA结合蛋白FMRP的互作界面，阐明了Caprin2在神经元mRNA转运复合体组装中的分子机制。
4. **文献名称**: *"Recombinant Caprin2 as a Potential Biomarker in Colorectal Cancer Progression"*
**作者**: Chen, H., et al. (2021)
**摘要**: 研究通过表达重组Caprin2蛋白制备多克隆抗体，发现其在结直肠癌患者血清中水平显著升高，提示Caprin2可能作为癌症进展的生物标志物。
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**说明**：以上文献信息为假设性示例，实际研究中建议通过PubMed、Web of Science等数据库检索关键词“Caprin2 recombinant”或结合具体研究领域筛选文献。真实研究可能聚焦于Caprin2在细胞应激、RNA代谢或癌症中的分子机制。
**Background of Caprin2 Recombinant Protein**
Caprin2 (Cell cycle-associated protein 2) is a multifunctional protein involved in regulating cellular processes such as proliferation, mRNA translation, and stress granule dynamics. It interacts with RNA-binding proteins and signaling molecules, notably playing a role in the Wnt/β-catenin pathway by binding to Adenomatous polyposis coli (APC), thereby influencing cell migration and cancer progression. Dysregulation of Caprin2 has been linked to cancers (e.g., hepatocellular carcinoma, gastric cancer) and neurological disorders, highlighting its therapeutic and diagnostic potential.
Recombinant Caprin2 is engineered in vitro using expression systems like *E. coli* or mammalian cells, ensuring high purity and activity for research applications. Its production often includes affinity tags (e.g., His-tag) for simplified purification. This recombinant protein serves as a critical tool to study Caprin2’s interactions, signaling roles, and disease mechanisms. Studies utilize it to explore its impact on Wnt-driven processes, cancer metastasis, and neuronal functions. Additionally, it aids in screening therapeutic agents targeting Caprin2-associated pathways. By providing a controlled and abundant protein source, recombinant Caprin2 accelerates both basic research and drug development efforts.
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