| 纯度 | >90%SDS-PAGE. |
| 种属 | E.coli |
| 靶点 | CDH-1 |
| Uniprot No | Q01738 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-773aa |
| 氨基酸序列 | MLGRSLLALLPFVGLAFSQSASQFTDPTTGFQFTGITDPVHDVTYGFVFPPLATSGAQSTEFIGEVVAPIASKWIGIALGGAMNNDLLLVAWANGNQIVSSTRWATGYVQPTAYTGTATLTTLPETTINSTHWKWVFRCQGCTEWNNGGGIDVTSQGVLAWAFSNVAVDDPSDPQSTFSEHTDFGFFGIDYSTAHSANYQNYLNGDSGNPTTTSTKPTSTSSSVTTGPTVSATPYDYIIVGAGPGGIIAADRLSEAGKKVLLLERGGPSTKQTGGTYVAPWATSSGLTKFDIPGLFESLFTDSNPFWWCKDITVFAGCLVGGGTSVNGALYWYPNDGDFSSSVGWPSSWTNHAPYTSKLSSRLPSTDHPSTDGQRYLEQSFNVVSQLLKGQGYNQATINDNPNYKDHVFGYSAFDFLNGKRAGPVATYLQTALARPNFTFKTNVMVSNVVRNGSQILGVQTNDPTLGPNGFIPVTPKGRVILSAGAFGTSRILFQSGIGPTDMIQTVQSNPTAAAALPPQNQWINLPVGMNAQDNPSINLVFTHPSIDAYENWADVWSNPRPADAAQYLANQSGVFAGASPKLNFWRAYSGSDGFTRYAQGTVRPGAASVNSSLPYNASQIFTITVYLSTGIQSRGRIGIDAALRGTVLTPPWLVNPVDKTVLLQALHDVVSNIGSIPGLTMITPDVTQTLEEYVDAYDPATMNSNHWVSSTTIGSSPQSAVVDSNVKVFGTNNLFIVDAGIIPHLPTGNPQGTLMSAAEQAAAKILALAGGP |
| 预测分子量 | 82 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于CDH-1(E-cadherin)重组蛋白的3篇代表性文献及摘要概括：
1. **文献名称**：Recombinant E-cadherin mediates epithelial cell adhesion through homophilic binding
**作者**：Nose, A., et al.
**摘要**：该研究通过重组表达E-cadherin胞外结构域，证明其通过钙离子依赖的同源二聚化介导上皮细胞间黏附，揭示了E-cadherin在维持组织完整性中的分子机制。
2. **文献名称**：Expression and purification of functional recombinant human E-cadherin in Escherichia coli
**作者**：Chen, Y., et al.
**摘要**：开发了一种在大肠杆菌中高效表达可溶性重组人E-cadherin胞外结构域的方法，通过优化密码子和纯化策略获得具有生物活性的蛋白，适用于体外黏附抑制实验。
3. **文献名称**：Structural basis of cell-cell adhesion by E-cadherin
**作者**：Shapiro, L., et al.
**摘要**：通过X射线晶体学解析重组E-cadherin胞外域的三维结构，揭示了其钙依赖性黏附界面的分子细节，为理解癌症中E-cadherin功能失调提供了结构基础。
注：以上为示例性内容，实际引用时建议通过PubMed或Web of Science核对最新文献。若需具体领域(如肿瘤转移、蛋白工程)的文献，可进一步补充关键词限定。
CDH-1. also known as E-cadherin, is a calcium-dependent transmembrane glycoprotein belonging to the cadherin superfamily. It plays a critical role in mediating cell-cell adhesion, particularly in epithelial tissues, by forming adherens junctions that maintain tissue architecture and polarity. The protein consists of an extracellular domain with five cadherin repeats for homophilic binding, a transmembrane domain, and a highly conserved cytoplasmic domain that interacts with catenins (e.g., β-catenin) to link the actin cytoskeleton. This connection is essential for transmitting mechanical forces and regulating signaling pathways involved in cell differentiation, migration, and apoptosis.
Recombinant CDH-1 proteins are engineered using expression systems like mammalian cells or bacteria to produce purified, functional forms of the protein for research and therapeutic applications. These proteins retain key structural domains required for calcium-dependent adhesion and intracellular signaling. Studies using recombinant CDH-1 have advanced our understanding of its tumor-suppressive functions, as loss of CDH-1 expression or mutations are strongly associated with epithelial-mesenchymal transition (EMT), cancer metastasis, and aggressive tumor behavior in carcinomas (e.g., breast, gastric).
In drug discovery, recombinant CDH-1 serves as a tool to screen compounds aiming to restore cadherin-mediated adhesion in metastatic cancers or modulate Wnt/β-catenin signaling. Its role in maintaining epithelial integrity also makes it relevant in regenerative medicine and tissue engineering. Despite challenges in preserving its native conformation during production, recombinant CDH-1 remains pivotal for elucidating mechanisms underlying developmental biology, cancer progression, and cell adhesion-related pathologies.
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