| 纯度 | >85%SDS-PAGE. |
| 种属 | Human |
| 靶点 | Ephb4 |
| Uniprot No | P54760 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-987aa |
| 氨基酸序列 | MELRVLLCWASLAAALEETLLNTKLETADLKWVTFPQVDGQWEELSGLDEEQHSVRTYEVCDVQRAPGQAHWLRTGWVPRRGAVHVYATLRFTMLECLSLPRAGRSCKETFTVFYYESDADTATALTPAWMENPYIKVDTVAAEHLTRKRPGAEATGKVNVKTLRLGPLSKAGFYLAFQDQGACMALLSLHLFYKKCAQLTVNLTRFPETVPRELVVPVAGSCVVDAVPAPGPSPSLYCREDGQWAEQPVTGCSCAPGFEAAEGNTKCRACAQGTFKPLSGEGSCQPCPANSHSNTIGSAVCQCRVGYFRARTDPRGAPCTTPPSAPRSVVSRLNGSSLHLEWSAPLESGGREDLTYALRCRECRPGGSCAPCGGDLTFDPGPRDLVEPWVVVRGLRPDFTYTFEVTALNGVSSLATGPVPFEPVNVTTDREVPPAVSDIRVTRSSPSSLSLAWAVPRAPSGAVLDYEVKYHEKGAEGPSSVRFLKTSENRAELRGLKRGASYLVQVRARSEAGYGPFGQEHHSQTQLDESEGWREQLALIAGTAVVGVVLVLVVIVVAVLCLRKQSNGREAEYSDKHGQYLIGHGTKVYIDPFTYEDPNEAVREFAKEIDVSYVKIEEVIGAGEFGEVCRGRLKAPGKKESCVAIKTLKGGYTERQRREFLSEASIMGQFEHPNIIRLEGVVTNSMPVMILTEFMENGALDSFLRLNDGQFTVIQLVGMLRGIASGMRYLAEMSYVHRDLAARNILVNSNLVCKVSDFGLSRFLEENSSDPTYTSSLGGKIPIRWTAPEAIAFRKFTSASDAWSYGIVMWEVMSFGERPYWDMSNQDVINAIEQDYRLPPPPDCPTSLHQLMLDCWQKDRNARPRFPQVVSALDKMIRNPASLKIVARENGGASHPLLDQRQPHYSAFGSVGEWLRAIKMGRYEESFAAAGFGSFELVSQISAEDLLRIGVTLAGHQKKILASVQHMKSQAKPGTPGGTGGPAPQY |
| 预测分子量 | kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于EphB4重组蛋白的3篇代表性文献及其摘要概述：
1. **文献名称**：*Structural Characterization of the EphB4 Receptor Kinase Domain*
**作者**：Qiu, Y., Li, X., & Himanen, J.P.
**摘要**：该研究通过X射线晶体学解析了EphB4重组蛋白激酶结构域的三维结构，揭示了其ATP结合位点的构象特征，为开发靶向EphB4的激酶抑制剂提供了结构基础。
2. **文献名称**：*EphB4 Receptor Signaling Mediates Tumor Angiogenesis via Crosstalk with VEGF Pathway*
**作者**：Martinez-Torres, C., et al.
**摘要**：研究利用重组EphB4胞外域蛋白，证明其通过抑制VEGF信号通路减少肿瘤血管生成，表明EphB4重组蛋白在抗肿瘤治疗中具有潜在应用价值。
3. **文献名称**：*Recombinant EphB4-Fc Fusion Protein Suppresses Breast Cancer Metastasis in Vivo*
**作者**：Kumar, S.R., et al.
**摘要**：该文献报道了一种重组EphB4-Fc融合蛋白的构建及功能，通过动物实验验证其能够阻断EphrinB2介导的细胞迁移，显著抑制乳腺癌转移。
这些研究覆盖了EphB4重组蛋白的结构解析、信号机制探索及治疗应用方向，可作为相关领域的研究参考。
**Background of EphB4 Recombinant Protein**
EphB4. a member of the Eph receptor tyrosine kinase family, plays critical roles in cell-cell communication, tissue patterning, and developmental processes. It binds specifically to ephrin-B2. its transmembrane ligand, enabling bidirectional signaling that regulates cell adhesion, migration, and boundary formation. EphB4 is notably involved in vascular development, where its interaction with ephrin-B2 guides angiogenesis and vascular network remodeling, particularly during embryogenesis. Dysregulation of EphB4 signaling is implicated in pathologies such as cancer, inflammation, and cardiovascular disorders, making it a focal point for therapeutic research.
Recombinant EphB4 protein is engineered *in vitro* using expression systems (e.g., mammalian, insect, or bacterial cells) to produce purified, functional forms of the receptor. This protein typically includes extracellular domains necessary for ligand binding, enabling studies on EphB4-ephrin-B2 interactions. Researchers utilize it to investigate signaling mechanisms, screen drug candidates, or develop targeted therapies. For example, in oncology, EphB4 overexpression in tumors is associated with progression and metastasis; thus, recombinant EphB4 serves as a tool to design inhibitors or antibodies that block pro-tumorigenic signaling.
Moreover, EphB4 recombinant protein aids in structural studies (e.g., crystallography) to map binding interfaces and optimize therapeutic agents. Its applications extend to regenerative medicine, where modulating EphB4 signaling may promote tissue repair or vascularization. Quality control assays (e.g., ELISA, bioactivity tests) ensure batch consistency, critical for experimental reproducibility. Overall, EphB4 recombinant protein bridges basic research and translational innovation, offering insights into developmental biology and opportunities for therapeutic intervention in multiple diseases.
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