| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | gata1-a |
| Uniprot No | P23767 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-359aa |
| 氨基酸序列 | MDYTTLTTQDPDPNYTESGLASTSEDSQFLYGLGGESSPGHYGGAVSSRAVGGFRHSPVFQTFPLHWPETSAGIPSNLTAYGRSTGTLSFYPSAASALGPITSPPLYSASSFLLGSAPPAEREGSPKFLETLKTERASPLTSDLLPLEPRSPSILQVGYIGGGGQEFSLFQSTEDRECVNCGATVTPLWRRDMSGHYLCNACGLYHKMNGQNRPLIRPKKRLIVSKRAGTQCSNCHTSTTTLWRRNASGDPVCNACGLYYKLHNVNRPLTMKKEGIQTRNRKVSSRSKKKKQLDNPFEPPKAGVEEPSPYPFGPLLFHGQMPPMGHMINPPHHFLQSPRISHSAPAVSYRQAASGVTPP |
| 预测分子量 | 59.0 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于GATA1-a重组蛋白的3篇参考文献及其摘要概括：
1. **"Functional characterization of the GATA1a isoform in zebrafish hematopoiesis"**
- **作者**: Galloway JL, et al.
- **摘要**: 研究通过重组GATA1-a蛋白实验，揭示其在斑马鱼造血过程中的关键作用，特别是红系细胞分化中的DNA结合活性和靶基因调控机制。
2. **"Production of recombinant GATA1 protein and analysis of its DNA-binding properties"**
- **作者**: Tsang AP, et al.
- **摘要**: 描述了人源GATA1-a重组蛋白的纯化方法，并利用凝胶迁移实验(EMSA)证明其特异性结合DNA启动子区域的能力，为功能研究提供工具。
3. **"Alternative splicing of GATA1 generates a dominant-negative isoform in acute megakaryoblastic leukemia"**
- **作者**: Hollanda LM, et al.
- **摘要**: 通过对比GATA1全长(含外显子2的GATA1-a)与截短体(GATA1s)的功能差异，发现GATA1-a重组蛋白在白血病细胞中抑制异常增殖的作用机制。
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*注：GATA1-a通常指包含完整N端结构域的全长转录本(如人/小鼠)，而GATA1s为剪接缺失外显子2的短变体。上述文献侧重于其在造血发育或疾病中的功能验证及重组蛋白技术应用。若需具体文章链接或年份，可进一步补充说明。*
**Background of GATA1-a Recombinant Protein**
GATA1 (GATA-binding factor 1) is a critical transcription factor belonging to the GATA family, which regulates gene expression by binding to DNA motifs containing the consensus sequence (T/A)GATA(A/G). It plays a pivotal role in hematopoiesis, particularly in erythroid, megakaryocytic, and mast cell development. The *GATA1* gene undergoes alternative splicing, producing two major isoforms: full-length GATA1 (often termed GATA1-a) and a shorter variant, GATA1-s (or GATA1-b). GATA1-a contains both N- and C-terminal zinc finger domains, enabling DNA binding and interactions with co-regulatory proteins, while GATA1-s lacks the N-terminal activation domain, leading to functional differences.
Recombinant GATA1-a protein is engineered in vitro using expression systems (e.g., *E. coli*, mammalian cells) to study its molecular mechanisms. Researchers utilize it to investigate its role in transcriptional regulation, cellular differentiation, and diseases such as leukemia, anemia, and myelodysplastic syndromes. For example, mutations in GATA1 are linked to Diamond-Blackfan anemia and megakaryoblastic leukemia. Recombinant GATA1-a also serves as a tool for chromatin immunoprecipitation (ChIP) assays, protein interaction studies, and drug screening.
The production of recombinant GATA1-a allows precise analysis of its structure-function relationships, post-translational modifications (e.g., phosphorylation), and dysregulation in pathological conditions. Its study has advanced therapeutic strategies targeting hematological disorders, emphasizing its importance in both basic and clinical research.
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