| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | ECM31 |
| Uniprot No | P38122 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-312aa |
| 氨基酸序列 | MNIMKRQLCTSSKRFFSTAKNVVKYNTIQDIRNKYFTGTPLSMCTAYDFITATWVNKANCDLLLVGDSLAMTSLGYDSTITLSLNEFKYHVASVCRAEGSSMVVVDMPFGTFESGISDGLKNAIDIMKLDSKVTSVKVEVGSYTKDKYAMKFIEELCSRGIPVMAHIGLTPQKVHSLGGYKVQGSKSLLQMQELYETAMQLQKIGCWSILIECVPHKMAQFITSKLSVPTIGIGAGNGTSGQVLVISDLLGMQGDSVPKFVKQAVNMTDIATQGLKEYIASVEDRTFPERGTHTFKVKEDLWNEFLSSINEK |
| 预测分子量 | 38.3 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于ECM31重组蛋白的假设性参考文献示例(请注意，这些文献为虚构示例，实际研究需通过学术数据库验证)：
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1. **文献名称**: "Heterologous Expression and Functional Characterization of Recombinant ECM31 in *Pichia pastoris*"
**作者**: Smith A, Brown C, et al.
**摘要**: 本研究在毕赤酵母系统中成功表达并纯化了重组ECM31蛋白，证实其具有β-1.3-葡聚糖合成酶活性，并揭示了其在真菌细胞壁合成中的关键作用。
2. **文献名称**: "Optimization of ECM31 Recombinant Protein Production in *E. coli* for Structural Studies"
**作者**: Lee JH, Kim S, et al.
**摘要**: 通过优化大肠杆菌表达条件，获得高纯度ECM31重组蛋白，并利用圆二色谱分析其二级结构，为后续酶学机制研究奠定基础。
3. **文献名称**: "Crystal Structure of ECM31 Reveals a Novel Glycosyltransferase Fold"
**作者**: Zhang Y, Wang L, et al.
**摘要**: 首次解析了重组ECM31蛋白的晶体结构，发现其独特的底物结合域，为设计靶向细胞壁合成的抗真菌药物提供了结构依据。
4. **文献名称**: "ECM31 Recombinant Protein Enhances Stress Tolerance in *Candida albicans*"
**作者**: Patel R, Garcia M, et al.
**摘要**: 通过重组表达ECM31.证明其过表达显著提高白色念珠菌对细胞壁干扰剂的抗性，提示其在真菌应激响应中的调控功能。
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**注意**：以上文献为模拟示例，实际研究中请通过PubMed、Web of Science等平台检索关键词“ECM31 recombinant protein”或“ECM31 expression”获取真实文献。若研究较少，可扩展至ECM31的基因功能或相关蛋白家族分析。
ECM31 recombinant protein is a engineered form of a protein associated with extracellular matrix (ECM) regulation, designed to study cellular interactions, tissue remodeling, and disease mechanisms. The extracellular matrix plays a critical role in maintaining tissue structure, signaling, and homeostasis, with ECM proteins influencing cell adhesion, migration, and differentiation. ECM31. though not universally standardized in nomenclature, is often linked to specific functional domains or motifs involved in ECM organization, such as collagen-binding regions or glycosaminoglycan interactions. Its recombinant version is typically produced using expression systems like *E. coli*, yeast, or mammalian cells, enabling scalable production for research or therapeutic applications.
Recombinant ECM31 is utilized to investigate pathologies like fibrosis, cancer metastasis, or inflammatory disorders, where ECM dysregulation is a hallmark. For instance, it may serve as a tool to modulate cell-ECM interactions in vitro or evaluate therapeutic agents targeting ECM degradation or synthesis. Its purity and consistency, achieved through affinity tags (e.g., His-tag) and chromatography, ensure reproducibility in experiments. Studies may focus on its role in signaling pathways (e.g., TGF-β, integrins) or as a biomarker in tissue engineering. While detailed characterization (structure, post-translational modifications) depends on the expression host, mammalian systems are preferred for native-like folding. Current research emphasizes its potential in regenerative medicine or as a drug target, reflecting broader interests in ECM-targeted therapies.
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