| 纯度 | >95%SDS-PAGE. |
| 种属 | Human |
| 靶点 | ERH |
| Uniprot No | P84090 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-104aa |
| 氨基酸序列 | MGSSHHHHHHSSGLVPRGSHMGSMSHTILLVQPTKRPEGRTYADYESVNE CMEGVCKMYEEHLKRMNPNSPSITYDISQLFDFIDDLADLSCLVYRADTQ TYQPYNKDWIKEKIYVLLRRQAQQAGK |
| 预测分子量 | 15 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于ERH重组蛋白的3篇参考文献(模拟示例，非真实文献)：
1. **《Expression and purification of recombinant ERH protein for structural studies》**
- 作者：Zhang L, et al.
- 摘要：研究通过大肠杆菌表达系统成功表达并纯化ERH重组蛋白，利用X射线晶体学解析其三维结构，揭示其参与RNA代谢的关键结构域。
2. **《ERH interacts with the spliceosome complex: implications for gene regulation》**
- 作者：Wang Y, et al.
- 摘要：通过体外重组ERH蛋白与剪接体复合物的互作实验，发现ERH在pre-mRNA剪接中的调控功能，并验证其通过特定结构域与剪接因子结合。
3. **《Functional characterization of ERH in cancer cell proliferation》**
- 作者：Kim S, et al.
- 摘要：利用重组ERH蛋白进行细胞实验，证明其通过调控细胞周期相关基因(如p21和Cyclin D1)抑制肿瘤细胞增殖，提示潜在抗癌治疗靶点。
注：以上文献名为虚构示例，实际研究中建议通过PubMed或Web of Science以关键词“ERH recombinant protein”、“Enhancer of Rudimentary Homolog”检索真实文献。
**Background of ERH Recombinant Protein**
The Enhancer of Rudimentary Homolog (ERH) protein is a highly conserved, multifunctional protein found across eukaryotes, playing diverse roles in cellular processes such as gene regulation, cell cycle progression, and RNA metabolism. Structurally, ERH comprises a compact α/β fold, enabling interactions with various molecular partners, including transcription factors, RNA-binding proteins, and components of the microRNA machinery. Its involvement in critical pathways—such as the pyrimidine biosynthesis pathway, mRNA splicing, and cell cycle checkpoints—highlights its biological significance. Dysregulation of ERH has been linked to cancers, neurodevelopmental disorders, and viral infections, making it a subject of growing interest in biomedical research.
Recombinant ERH protein is produced using genetic engineering techniques, typically through heterologous expression in bacterial (e.g., *E. coli*) or mammalian systems. This approach ensures high purity and scalability for experimental or therapeutic applications. The recombinant protein retains native functional properties, enabling studies on its molecular mechanisms, such as its role in modulating the Wnt/β-catenin signaling pathway or its interaction with survival motor neuron (SMN) complexes.
In drug discovery, ERH recombinant protein serves as a tool for screening inhibitors or stabilizers targeting its activity in diseases like glioblastoma or hepatitis B. Additionally, it aids structural biology efforts, including crystallography and NMR, to elucidate binding interfaces for rational drug design. Challenges remain in optimizing post-translational modifications and solubility for certain applications, but advances in expression systems continue to enhance its utility. Overall, ERH recombinant protein bridges fundamental research and translational medicine, offering insights into cellular homeostasis and therapeutic opportunities.
×
