| 纯度 | >90%SDS-PAGE. |
| 种属 | E.coli |
| 靶点 | isaA |
| Uniprot No | P60157 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 30-233aa |
| 氨基酸序列 | AEVNVDQAHLVDLAHNHQDQLNAAPIKDGAYDIHFVKDGFQYNFTSNGTTWSWSYEAANGQTAGFSNVAGADYTTSYNQGSNVQSVSYNAQSSNSNVEAVSAPTYHNYSTSTTSSSVRLSNGNTAGATGSSAAQIMAQRTGVSASTWAAIIARESNGQVNAYNPSGASGLFQTMPGWGPTNTVDQQINAAVKAYKAQGLGAWGF |
| 预测分子量 | 28.8 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于isaA重组蛋白的假设性参考文献示例(请注意，文献为虚构，仅供参考格式)：
1. **文献名称**: "Heterologous Expression and Functional Analysis of Staphylococcus aureus IsaA as a Recombinant Protein"
**作者**: Müller R, et al.
**摘要**: 本研究在大肠杆菌中成功表达并纯化了重组IsaA蛋白，证实其具有细胞壁水解酶活性，且该活性在金黄色葡萄球菌自溶过程中起关键作用，为理解细菌分裂机制提供了新见解。
2. **文献名称**: "Recombinant IsaA Protein Enhances β-Lactam Antibiotic Susceptibility in Methicillin-Resistant Staphylococcus aureus (MRSA)"
**作者**: Chen L, et al.
**摘要**: 通过体外实验证明重组IsaA蛋白能破坏MRSA细胞壁完整性，显著提高青霉素类抗生素的杀菌效果，提示其作为抗生素佐剂的潜在应用价值。
3. **文献名称**: "Crystallization and Enzymatic Characterization of the Recombinant IsaA Lytic Transglycosylase from S. aureus"
**作者**: Tanaka K, et al.
**摘要**: 首次报道重组IsaA蛋白的结晶结构，揭示其活性位点特征，并通过定点突变验证了关键氨基酸对其肽聚糖水解功能的影响，为抑制剂设计奠定基础。
4. **文献名称**: "Immunomodulatory Effects of Recombinant IsaA in a Murine Model of Staphylococcal Infection"
**作者**: González S, et al.
**摘要**: 动物实验表明，注射重组IsaA蛋白可激活巨噬细胞并促进细菌清除，同时调节促炎细胞因子水平，提示其兼具直接抗菌和免疫调节双重功能。
**备注**：以上内容为模拟数据，实际文献需通过PubMed、Google Scholar等平台检索关键词如"recombinant IsaA protein"或"Staphylococcus aureus IsaA expression"获取。建议结合具体研究方向补充筛选条件(如年限、物种)。
**Background of isaA Recombinant Protein**
The *isaA* gene, originally identified in *Staphylococcus aureus*, encodes an immunodominant antigen A (IsaA), a putative lytic transglycosylase involved in cell wall metabolism. As a member of the autolysin family, IsaA plays a role in bacterial cell wall remodeling, peptidoglycan hydrolysis, and daughter cell separation during division. Its enzymatic activity contributes to the dynamic restructuring of the cell wall, a process critical for bacterial growth, virulence, and adaptation to environmental stress.
IsaA has garnered attention due to its immunogenic properties and potential role in *S. aureus* pathogenesis. Studies suggest it may help the pathogen evade host immune defenses by modulating cell surface properties or interacting with host molecules. Additionally, IsaA is implicated in biofilm formation, a key factor in chronic infections and antibiotic resistance. Its surface-exposed nature and conserved sequence across *S. aureus* strains make it a candidate for diagnostic or therapeutic targeting.
Recombinant IsaA protein is typically produced using heterologous expression systems, such as *Escherichia coli*, to enable large-scale purification for research or clinical applications. The recombinant form retains functional domains, including its enzymatic active site and putative peptidoglycan-binding motifs, allowing studies on its biochemical activity, structure-function relationships, and interactions with host immune components. Researchers also explore its potential as a vaccine antigen or a target for monoclonal antibodies.
Despite progress, challenges remain, such as understanding its regulatory mechanisms in vivo and reconciling its dual roles in bacterial survival and host immune activation. Ongoing research aims to clarify its contribution to staphylococcal virulence and assess its utility in combating antibiotic-resistant infections.
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