| 纯度 | >90%SDS-PAGE. |
| 种属 | E.coli |
| 靶点 | entB |
| Uniprot No | P0ADI4 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-285aa |
| 氨基酸序列 | MAIPKLQAYA LPESHDIPQN KVDWAFEPQR AALLIHDMQD YFVSFWGENC PMMEQVIANI AALRDYCKQH NIPVYYTAQP KEQSDEDRAL LNDMWGPGLT RSPEQQKVVD RLTPDADDTV LVKWRYSAFH RSPLEQMLKE SGRNQLIITG VYAHIGCMTT ATDAFMRDIK PFMVADALAD FSRDEHLMSL KYVAGRSGRV VMTEELLPAP IPASKAALRE VILPLLDESD EPFDDDNLID YGLDSVRMMA LAARWRKVHG DIDFVMLAKN PTIDAWWKLL SREVK |
| 预测分子量 | 32,5 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于entB重组蛋白的3-4篇代表性文献的简要整理：
1. **"Cloning and expression of the entB gene involved in enterobactin biosynthesis in Escherichia coli"**
- **作者**: K. Raymond 等
- **摘要**: 研究通过克隆entB基因并在大肠杆菌中重组表达，验证其编码的异分支酸酯酶活性，揭示其在肠菌素(enterobactin)铁载体合成中的关键作用。
2. **"Structural characterization of the entB-encoded enzyme in the siderophore pathway of E. coli"**
- **作者**: A.M. Gehring 等
- **摘要**: 报道了重组表达的EntB蛋白的晶体结构解析，阐明了其作为双功能酶(异分支酸酯酶/铁载体组装酶)的催化机制及底物结合位点。
3. **"Functional analysis of EntB in microbial iron acquisition systems"**
- **作者**: J.H. Crosa 等
- **摘要**: 通过基因敲除和重组蛋白回补实验，证明EntB在病原菌铁摄取途径中的必要性，并探讨其作为抗菌治疗靶点的潜力。
4. **"Recombinant EntB production and its application in siderophore inhibition assays"**
- **作者**: C.M. Perry 等
- **摘要**: 优化了EntB重组蛋白在大肠杆菌中的可溶性表达与纯化工艺，并利用该蛋白开发铁载体合成抑制实验，用于筛选潜在抗菌化合物。
*注：以上文献信息为示例性质，具体内容需根据实际发表的论文调整。建议通过PubMed或SciFinder以“entB recombinant protein”为关键词检索近年文献获取准确信息。*
EntB is a key enzyme involved in the biosynthesis of enterobactin, a high-affinity iron-chelating siderophore produced by *Escherichia coli* and other Gram-negative bacteria. As part of the enterobactin synthetase complex (EntABCDEF), EntB functions as a dual-role enzyme with both aryl carrier protein (ArCP) and isochorismatase activities. Its primary role is to facilitate iron acquisition under iron-limited conditions, a critical survival strategy for pathogenic bacteria during host infection. The ArCP domain of EntB activates and shuttles intermediates during enterobactin assembly, while its isochorismatase domain hydrolyzes isochorismate to generate 2.3-dihydroxybenzoate (DHB), a precursor for enterobactin synthesis.
Recombinant EntB protein is typically produced using heterologous expression systems, such as *E. coli* strains (e.g., BL21(DE3)), through cloning and overexpression of the *entB* gene. This engineered protein serves as a valuable tool for studying bacterial iron metabolism, siderophore-mediated virulence, and potential antimicrobial targets. Its structural and functional characterization has provided insights into the molecular mechanisms of nonribosomal peptide synthetase (NRPS)-independent siderophore pathways. Researchers also explore EntB's enzymatic properties for biotechnological applications, including enzyme engineering and inhibitor development to disrupt bacterial iron acquisition. Recent studies focus on EntB's role in microbial competition, its potential as a drug target, and its use in synthetic biology for designing novel metalloenzymes or biosensors. The soluble, purified recombinant EntB enables crystallographic studies and high-throughput screening of antibacterial compounds targeting iron-scavenging pathways.
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