| 纯度 | >90%SDS-PAGE. |
| 种属 | E.coli |
| 靶点 | RBP47A |
| Uniprot No | F4I3B3 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-445aa |
| 氨基酸序列 | MQTPNNNGSTDSVLPPTSAGTTPPPPLQQSTPPPQQQQQQQWQQQQQWMAAMQQYPAAAMAMMQQQQMMMYPHPQYAPYNQAAYQQHPQFQYAAYQQQQQQHHQSQQQPRGGSGGDDVKTLWVGDLLHWMDETYLHTCFSHTNEVSSVKVIRNKQTCQSEGYGFVEFLSRSAAEEALQSFSGVTMPNAEQPFRLNWASFSTGEKRASENGPDLSIFVGDLAPDVSDAVLLETFAGRYPSVKGAKVVIDSNTGRSKGYGFVRFGDENERSRAMTEMNGAFCSSRQMRVGIATPKRAAAYGQQNGSQALTLAGGHGGNGSMSDGESNNSTIFVGGLDADVTEEDLMQPFSDFGEVVSVKIPVGKGCGFVQFANRQSAEEAIGNLNGTVIGKNTVRLSWGRSPNKQWRSDSGNQWNGGYSRGQGYNNGYANQDSNMYATAAAAVPGAS |
| 预测分子量 | 53.5 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于拟南芥RBP47A(RNA结合蛋白)重组蛋白的3篇代表性参考文献示例，供参考：
---
1. **文献名称**: "Arabidopsis RBP47A regulates ABA signaling by stabilizing stress granules under drought stress"
**作者**: Wang, X., et al.
**摘要**: 研究通过重组RBP47A蛋白验证其RNA结合活性，发现其在干旱胁迫下与ABA受体互作，促进应激颗粒组装，从而增强植物的抗旱性。体外实验表明重组蛋白优先结合富含AU的mRNA区域。
---
2. **文献名称**: "Recombinant RBP47A enhances freezing tolerance by modulating RNA metabolism in vitro"
**作者**: Li, S., et al.
**摘要**: 利用大肠杆菌表达系统纯化RBP47A重组蛋白，证实其具有低温诱导的RNA伴侣功能。体外实验显示蛋白可防止目标mRNA在冷胁迫下形成二级结构，为解析其抗冻分子机制提供依据。
---
3. **文献名称**: "Structural and functional analysis of RBP47A in seed dormancy regulation"
**作者**: Nakamura, T., et al.
**摘要**: 通过晶体结构解析发现重组RBP47A的RRM结构域特异性识别种子萌发相关基因的3'UTR。突变实验表明其磷酸化修饰影响蛋白与P体成分的相互作用，进而调控种子休眠的ABA依赖性通路。
---
**提示**：若需真实文献，建议在PubMed等平台以“RBP47A recombinant”或“Arabidopsis RNA-binding protein 47A”为关键词检索，并优先筛选近5年植物分子生物学领域的论文。部分研究可能使用别名(如AtRBP47A)或聚焦于其旁系同源蛋白。
**Background of RBP47A Recombinant Protein**
RBP47A (RNA-Binding Protein 47A) is a member of the RNA-binding protein family, which plays critical roles in post-transcriptional gene regulation, including RNA stability, splicing, transport, and translation. Initially identified in plants (e.g., *Arabidopsis thaliana*), RBP47A is associated with stress responses, particularly under abiotic stresses such as drought, heat, or high salinity. It is hypothesized to function as a molecular chaperone, binding to RNA molecules during stress to prevent misfolding or degradation, thereby aiding cellular recovery.
The recombinant RBP47A protein is engineered to enable detailed functional studies. Produced via heterologous expression systems (e.g., *E. coli* or mammalian cell lines*), the protein is purified for in vitro analyses. Its recombinant form often includes tags (e.g., His-tag) for simplified purification and detection. Researchers utilize this protein to investigate RNA-protein interactions, stress granule dynamics, and mechanisms of stress adaptation.
Studies on RBP47A have expanded into biomedical and agricultural research, given its conserved RNA-binding domains and potential cross-species relevance. For example, in crop engineering, modulating RBP47A expression may enhance stress tolerance. In human cells, homologs of RNA-binding proteins are linked to diseases like neurodegeneration or cancer, making RBP47A a comparative model for understanding conserved RNA-regulatory pathways.
Overall, RBP47A recombinant protein serves as a vital tool for dissecting RNA biology and stress response mechanisms, bridging fundamental research with biotechnological applications.
×
