| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | DCTN1 |
| Uniprot No | Q14203 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 213-547aa |
| 氨基酸序列 | PSKEEEGLRAQVRDLEEKLETLRLKRAEDKAKLKELEKHKIQLEQVQEWKSKMQEQQADLQRRLKEARKEAKEALEAKERYMEEMADTADAIEMATLDKEMAEERAESLQQEVEALKERVDELTTDLEILKAEIEEKGSDGAASSYQLKQLEEQNARLKDALVRMRDLSSSEKQEHVKLQKLMEKKNQELEVVRQQRERLQEELSQAESTIDELKEQVDAALGAEEMVEMLTDRNLNLEEKVRELRETVGDLEAMNEMNDELQENARETELELREQLDMAGARVREAQKRVEAAQETVADYQQTIKKYRQLTAHLQDVNRELTNQQEASVERQQQ |
| 预测分子量 | 55.2 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于DCTN1重组蛋白的3篇参考文献示例(注：部分文献信息为示例性概括，实际引用请核实具体来源)：
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1. **文献名称**: "Dynactin subunit p150(glued) is essential for retrograde axonal transport in Drosophila"
**作者**: Puls, I., et al.
**摘要**: 该研究利用重组DCTN1蛋白(p150)在果蝇模型中分析其功能，发现DCTN1缺失导致逆向轴突运输缺陷，证实其在动力蛋白-肌球蛋白复合体中的关键作用，并关联到神经退行性病变机制。
2. **文献名称**: "Structural insights into the microtubule-binding domain of dynactin p150(glued)"
**作者**: Carter, A.P., et al.
**摘要**: 通过表达并纯化重组DCTN1蛋白的微管结合域，结合X射线晶体学解析其三维结构，揭示了其与微管相互作用的关键氨基酸残基，为理解运输复合体的组装机制提供结构基础。
3. **文献名称**: "DCTN1 mutations impair mitochondrial trafficking in iPSC-derived neurons from ALS patients"
**作者**: Smith, B.N., et al.
**摘要**: 研究利用重组DCTN1突变体蛋白，在诱导多能干细胞分化的神经元中模拟肌萎缩侧索硬化症(ALS)，发现突变导致线粒体运输障碍，提示DCTN1功能异常在神经退行中的直接作用。
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**备注**：以上文献标题及内容为示例性概括，实际研究中请通过学术数据库(如PubMed、Web of Science)以关键词"DCTN1 recombinant"或"dynactin p150"检索最新文献，并优先选择高影响力期刊论文。
**Background of DCTN1 Recombinant Protein**
DCTN1 (Dynactin subunit 1), also known as p150(glued), is a critical component of the dynactin complex, a multi-subunit protein assembly required for dynein-mediated intracellular transport. This protein plays a central role in regulating microtubule-dependent cytoplasmic dynein motor activity, facilitating retrograde transport of vesicles, organelles, and molecular cargo along microtubule networks. Structurally, DCTN1 contains an N-terminal cytoskeleton-associated protein glycine-rich (CAP-Gly) domain, which mediates interactions with microtubules and post-translational modifications like tubulin tyrosination, and a C-terminal domain that binds dynein and other dynactin subunits.
Dysregulation of DCTN1 is implicated in neurodegenerative disorders, including Perry syndrome, amyotrophic lateral sclerosis (ALS), and Parkinson’s disease, as well as cancer metastasis, due to disrupted intracellular trafficking. Recombinant DCTN1 protein, produced via heterologous expression systems (e.g., *E. coli* or mammalian cells), enables *in vitro* studies to dissect its molecular interactions, structural dynamics, and functional roles. It is widely used in binding assays, structural biology (e.g., cryo-EM or X-ray crystallography), and drug discovery targeting dynein-dynactin pathways. Purification often involves affinity tags (e.g., His-tag) for high yield and purity. Research on DCTN1 recombinant protein enhances understanding of cellular transport mechanisms and disease pathogenesis, offering potential therapeutic avenues for neurodegeneration and cancer.
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