| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | RBBP7 |
| Uniprot No | Q16576 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-425aa |
| 氨基酸序列 | MASKEMFEDTVEERVINEEYKIWKKNTPFLYDLVMTHALQWPSLTVQWLP EVTKPEGKDYALHWLVLGTHTSDEQNHLVVARVHIPNDDAQFDASHCDSD KGEFGGFGSVTGKIECEIKINHEGEVNRARYMPQNPHIIATKTPSSDVLV FDYTKHPAKPDPSGECNPDLRLRGHQKEGYGLSWNSNLSGHLLSASDDHT VCLWDINAGPKEGKIVDAKAIFTGHSAVVEDVAWHLLHESLFGSVADDQK LMIWDTRSNTTSKPSHLVDAHTAEVNCLSFNPYSEFILATGSADKTVALW DLRNLKLKLHTFESHKDEIFQVHWSPHNETILASSGTDRRLNVWDLSKIG EEQSAEDAEDGPPELLFIHGGHTAKISDFSWNPNEPWVICSVSEDNIMQI WQMAENIYNDEESDVTTSELEGQGS |
| 预测分子量 | 73 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是3篇关于RBBP7重组蛋白研究的示例参考文献(内容为模拟概括，非真实文献)：
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1. **标题**: *Recombinant RBBP7 Expression and Its Role in Histone Binding*
**作者**: Zhang Y, et al.
**摘要**: 研究利用大肠杆菌系统表达并纯化重组RBBP7蛋白，通过体外结合实验证实其与组蛋白H3-H4复合物的特异性相互作用，揭示了RBBP7在染色质组装中的分子机制。
2. **标题**: *Structural Insights into RBBP7 WD40 Domain via X-ray Crystallography*
**作者**: Lee S, et al.
**摘要**: 解析了重组RBBP7蛋白的晶体结构，发现其WD40结构域形成典型的β-螺旋结构，并鉴定了与组蛋白去乙酰化酶复合物(HDAC)结合的关键氨基酸位点，为靶向表观遗传治疗提供依据。
3. **标题**: *Functional Analysis of Recombinant RBBP7 in Chromatin Remodeling Complex Assembly*
**作者**: Patel R, et al.
**摘要**: 开发了体外重组RBBP7与NuRD复合体核心蛋白的共表达体系，证明RBBP7通过调控复合体稳定性影响染色质重塑活性，并应用于癌症细胞模型的表观遗传沉默研究。
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**提示**：若需真实文献，建议通过PubMed或Google Scholar以关键词“RBBP7 recombinant protein”或“RBBP7 purification”检索，并筛选涉及蛋白表达、结构或功能机制的论文。
RBBP7 (Retinoblastoma-Binding Protein 7), also known as RbAp46. is a conserved eukaryotic protein belonging to the WD40 repeat protein family. It plays a critical role in chromatin remodeling and epigenetic regulation by interacting with histone complexes and chromatin-modifying enzymes. Structurally, it contains multiple WD40 domains that facilitate protein-protein interactions, enabling its integration into multi-subunit complexes like the NuRD (Nucleosome Remodeling and Deacetylase) complex and PRC2 (Polycomb Repressive Complex 2). These complexes are essential for histone deacetylation, DNA methylation, and gene silencing, influencing cellular processes such as cell cycle regulation, differentiation, and apoptosis.
Recombinant RBBP7 is produced in vitro using expression systems (e.g., E. coli or mammalian cells) to study its molecular functions without endogenous interference. Its recombinant form retains the ability to bind histones H3 and H4. making it valuable for investigating chromatin dynamics, histone modification crosstalk, and epigenetic inheritance. Studies link RBBP7 dysregulation to cancer progression, neurodegenerative diseases, and developmental disorders, positioning it as a potential therapeutic target. For example, its overexpression in certain cancers correlates with aberrant cell proliferation, while reduced levels may impair neuronal development.
Researchers utilize recombinant RBBP7 in assays to dissect its role in DNA repair, X-chromosome inactivation, and stem cell pluripotency. It also serves as a molecular tool for drug screening aimed at modulating epigenetic enzymes. Quality-controlled batches ensure consistency in biochemical studies, with purity and activity validated via SDS-PAGE, western blotting, and binding assays. Overall, RBBP7 recombinant protein bridges structural biology and disease mechanism research, offering insights into epigenetics-driven pathologies.
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