| 纯度 | >90%SDS-PAGE. |
| 种属 | E.coli |
| 靶点 | ATP5MG |
| Uniprot No | Q28852 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 2-103aa |
| 氨基酸序列 | AEFVRNLAEKAPALVNAAVTYSKPRLATFWYYAKVELVPPTPAEIPTAIQSLKKIINSAKTGSFKQLTVKEALLNGLVATEVWMWFYVGEIIGKRGIIGYDV |
| 预测分子量 | 18.7 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于ATP5MG重组蛋白的3条示例参考文献(内容为虚构示例，建议通过学术数据库验证真实文献)：
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1. **文献名称**: *Structural and Functional Analysis of ATP5MG in Mitochondrial ATP Synthase*
**作者**: Zhang L, et al.
**摘要**: 本研究解析了ATP5MG重组蛋白的晶体结构，揭示了其作为ATP合酶亚基在质子通道形成中的作用，并通过敲除实验证明其缺失导致线粒体膜电位异常和能量代谢障碍。
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2. **文献名称**: *ATP5MG Recombinant Protein Enhances Neuronal Survival in Alzheimer's Disease Models*
**作者**: Kim S, et al.
**摘要**: 通过在大鼠神经元细胞中过表达ATP5MG重组蛋白，发现其可减少β-淀粉样蛋白诱导的线粒体氧化应激，改善ATP生成，为神经退行性疾病治疗提供潜在靶点。
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3. **文献名称**: *Development of a High-Yield Expression System for ATP5MG Recombinant Protein in E. coli*
**作者**: Müller R, et al.
**摘要**: 报道了一种基于大肠杆菌的ATP5MG重组蛋白高效表达与纯化方法，优化后的蛋白具有天然构象和酶活性，适用于体外线粒体功能研究。
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建议通过 **PubMed/Google Scholar** 搜索关键词 "ATP5MG recombinant protein" 或 "ATP synthase subunit g" 获取真实文献。
ATP5MG, also known as ATP synthase membrane subunit g or subunit 6.8. is a nuclear-encoded component of mitochondrial ATP synthase (Complex V), which plays a central role in cellular energy production. This protein is part of the Fo complex, a transmembrane proton channel within the mitochondrial inner membrane. ATP5MG contributes to the structural stability and proton translocation activity of ATP synthase, facilitating the conversion of ADP to ATP during oxidative phosphorylation. The human ATP5MG gene is located on chromosome 16 and produces multiple transcript variants through alternative splicing.
Research has linked ATP5MG to mitochondrial function regulation and cellular metabolism. Its expression varies across tissues, with higher levels in energy-demanding organs like the heart and brain. Dysregulation of ATP5MG has been implicated in mitochondrial disorders, neurodegenerative diseases, and cancer progression. In Alzheimer's disease, reduced ATP5MG levels correlate with impaired mitochondrial function and neuronal loss. Cancer studies suggest its overexpression may promote tumor growth by enhancing ATP production in hypoxic microenvironments.
Recombinant ATP5MG protein is typically produced using prokaryotic (e.g., E. coli) or eukaryotic expression systems to preserve post-translational modifications. The purified protein serves as a valuable tool for structural studies, enzyme activity assays, and antibody production. Researchers utilize it to investigate ATP synthase assembly, mitochondrial bioenergetics, and disease mechanisms. In therapeutic development, recombinant ATP5MG aids in screening compounds targeting mitochondrial dysfunction. Recent applications include its use in generating cellular models of mitochondrial disorders and studying metabolic adaptation in cancer cells.
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