| 纯度 | >90%SDS-PAGE. |
| 种属 | Mouse |
| 靶点 | Klra3 |
| Uniprot No | Q64329 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 70-266aa |
| 氨基酸序列 | QYNQHKQEINETLNHHHNCSNMQRAFNLKEEMLTNKSIDCRPSNETLEYIKREQDRWDSKTKTVLDSSRDTGRGVKYWFCYSTKCYYFIMNKTTWSGCKANCQHYSVPILKIEDEDELKFLQRHVIPENYWIGLSYDKKKKEWAWIDNGPSKLDMKIRKMNFKSRGCVFLSKARIEDIDCNIPYYCICGKKLDKFPD |
| 预测分子量 | 27.6 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于Klra3重组蛋白的3-4篇模拟参考文献(文献为虚构示例，供参考)：
1. **文献名称**：*"Structural and functional characterization of recombinant Klra3 in NK cell-mediated cytotoxicity"*
**作者**：Smith, A.B. et al.
**摘要**：研究通过重组Klra3蛋白表达，解析其与H-2Dᵏ MHC I类分子的结合机制，揭示其在自然杀伤细胞识别靶细胞中的关键作用。
2. **文献名称**：*"Klra3 recombinant protein engineering for ligand specificity analysis"*
**作者**：Wang, C. & Kane, K.P.
**摘要**：利用重组Klra3蛋白进行突变实验，鉴定其胞外结构域中决定配体选择性的关键氨基酸残基，为免疫受体设计提供依据。
3. **文献名称**：*"Recombinant Klra3 binds viral peptides and modulates antiviral response in mice"*
**作者**：Li, X. et al.
**摘要**：证明重组Klra3蛋白通过结合病毒抗原肽段激活NK细胞，在流感病毒感染小鼠模型中增强早期免疫防御。
4. **文献名称**：*"Crystallographic study of recombinant Klra3-MHC complex"*
**作者**：Braud, V.M. et al.
**摘要**：通过X射线晶体学解析重组Klra3蛋白与MHC-I的复合物结构，阐明其识别模式及免疫突触形成的分子基础。
注：以上文献为模拟生成，实际研究中请通过PubMed或学术数据库检索真实文献。
**Background of Klra3 Recombinant Protein**
Klra3 (Killer cell lectin-like receptor A3), also known as Ly49A3 in mice, belongs to the C-type lectin-like receptor family expressed primarily on natural killer (NK) cells and subsets of T lymphocytes. These receptors play critical roles in immune regulation by recognizing major histocompatibility complex (MHC) class I molecules on target cells, thereby modulating NK cell activation or inhibition. Klra3 is a member of the Ly49 multigene family, which exhibits significant polymorphism and haplotype variability across mouse strains, influencing ligand specificity and immune responses.
Recombinant Klra3 protein is engineered to study its structure, ligand interactions, and functional mechanisms *in vitro*. It is typically produced using expression systems (e.g., bacterial, insect, or mammalian cells) to ensure proper folding and post-translational modifications. The recombinant form often includes extracellular domains responsible for MHC-I binding, enabling researchers to investigate its role in immune tolerance, viral infection responses, and tumor surveillance.
Studies using Klra3 recombinant protein have elucidated its interaction with H-2Dᵏ and other MHC-I ligands, shedding light on how NK cells discriminate between healthy and stressed cells. Additionally, it serves as a tool for developing antibodies, blocking assays, or structural studies (e.g., crystallography). Its polymorphic nature also makes it a model for understanding receptor evolution and adaptive immunity. Challenges in Klra3 research include reconciling its *in vitro* binding data with *in vivo* functional outcomes and addressing species-specific differences when translating findings to human NK receptors like KIRs.
Overall, Klra3 recombinant protein remains pivotal in dissecting NK cell biology, with implications for immunotherapy, infectious disease, and cancer research.
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